Recombinant E.coli, G/U mismatch-specific DNA glycosylase; xanthine DNA glycosylase, His-tagged
Cat.No. : | mug-542E |
Product Overview : | mug, 1-168aa, E.coli, His tag, E.coli |
- Specification
- Gene Information
- Related Products
- Download
Description : | G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Recombinant E.coli mug protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Source : | E.coli |
Species : | E.coli, |
Tag : | His-tag |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol |
Molecular Mass : | 21.1 kDa (191aa) confirmed by MALDI-TOF |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R |
Purity : | >90% by SDS - PAGE |
Storage : | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5 mg/ml (determined by Bradford assay) |
Gene Name : | mug?G/U mismatch-specific DNA glycosylase; xanthine DNA glycosylase [?Escherichia coli str. K-12 substr. MG1655?] |
Official Symbol : | mug |
Synonyms : | mug; dug; ECK3058; JW3040; ygjF; G/U mismatch-specific DNA glycosylase; xanthine DNA glycosylase; G/U mismatch-specific DNA glycosylase; xanthine DNA glycosylase; NP_417540.1; EC 3.2.2.- |
Gene ID : | 947560 |
Protein Refseq : | NP_417540 |
Pathway : | Base Excision Repair; Base-Excision Repair, AP Site Formation; Depurination |
Products Types
◆ Recombinant Protein | ||
mug-01E | Recombinant E. coli mug Protein, His-tagged | +Inquiry |
mug-1549E | Recombinant E. coli (strain K12) mug Protein (Met1-Arg168) | +Inquiry |
mug-93E | Recombinant E.coli Mug, His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (0)
Ask a questionAsk a Question for All mug Products
Required fields are marked with *
My Review for All mug Products
Required fields are marked with *
0
Inquiry Basket