Recombinant Escherichia coli (strain K12) hldE protein, His-SUMO-tagged

• Cat.No.: hldE-3708E
• Product Overview: Recombinant Escherichia coli (strain K12) hldE protein(P76658)(1-477aa), fused to N-terminal His-SUMO tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Escherichia coli (strain K12)
• Tag: His-SUMO
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 67.1 kDa
• Protein length: 1-477aa
• AA Sequence: MKVTLPEFERAGVMVVGDVMLDRYW YGPTSRISPEAPVPVVKVNTIEERP GGAANVAMNIASLGANARLVGLTGI DDAARALSKSLADVNVKCDFVSVPT HPTITKLRVLSRNQQLIRLDFEEGF EGVDPQPLHERINQALSSIGALVLS DYAKGALASVQQMIQLARKAGVPVL IDPKGTDFERYRGATLLTPNLSEFE AVVGKCKTEEEIVERGMKLIADYEL SALLVTRSEQGMSLLQPGKAPLHMP TQAQEVYDVTGAGDTVIGVLAATLA AGNSLEEACFFANAAAGVVVGKLGT STVSPIELENAVRGRADTGFGVMTE EELKLAVAAARKRGEKVVMTNGVFD ILHAGHVSYLANARKLGDRLIVAVN SDASTKRLKGDSRPVNPLEQRMIVL GALEAVDWVVSFEEDTPQRLIAGIL PDLLVKGGDYKPEEIAGSKEVWANG GEVLVLNFEDGCSTTNIIKKIQQDK KG
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.