Recombinant Escherichia coli UVRD Protein (1-720 aa)

• Cat.No.: UVRD-2458E
• Product Overview: Recombinant Escherichia coli (strain K12) UVRD Protein (1-720 aa) is produced by E. coli expression system. Protein Description: Full Length.

Specification

• Description: A helicase with DNA-dependent ATPase activity. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand. Initiates unwinding more efficiently from a nicked substrate than ds duplex DNA. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA.
• Source: E. coli
• Species: E. coli
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 82.0 kDa
• Protein length: 1-720 aa
• AA Sequence: MDVSYLLDSLNDKQREAVAAPRSNL LVLAGAGSGKTRVLVHRIAWLMSVE NCSPYSIMAVTFTNKAAAEMRHRIG QLMGTSQGGMWVGTFHGLAHRLLRA HHMDANLPQDFQILDSEDQLRLLKR LIKAMNLDEKQWPPRQAMWYINSQK DEGLRPHHIQSYGNPVEQTWQKVYQ AYQEACDRAGLVDFAELLLRAHELW LNKPHILQHYRERFTNILVDEFQDT NNIQYAWIRLLAGDTGKVMIVGDDD QSIYGWRGAQVENIQRFLNDFPGAE TIRLEQNYRSTSNILSAANALIENN NGRLGKKLWTDGADGEPISLYCAFN ELDEARFVVNRIKTWQDNGGALAEC AILYRSNAQSRVLEEALLQASMPYR IYGGMRFFERQEIKDALSYLRLIAN RNDDAAFERVVNTPTRGIGDRTLDV VRQTSRDRQLTLWQACRELLQEKAL AGRAASALQRFMELIDALAQETADM PLHVQTDRVIKDSGLRTMYEQEKGE KGQTRIENLEELVTATRQFSYNEED EDLMPLQAFLSHAALEAGEGQADTW QDAVQLMTLHSAKGLEFPQVFIVGM EEGMFPSQMSLDEGGRLEEERRLAY VGVTRAMQKLTLTYAETRRLYGKEV YHRPSRFIGELPEECVEEVRLRATV SRPVSHQRMGTPMVENDSGYKLGQR VRHAKFGEGTIVNMEGSGEHSRLQV AFQGQGIKWLVAAYARLESV
• Purity: > 85% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: uvrD ssDNA translocase and dsDNA helicase - DNA helicase II [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: UVRD
• Synonyms: uvrD; dar-2; dda; ECK3808; mutU; pdeB; rad; recL; srjC; uvr502; uvrE
• Gene ID: 948347
• Protein Refseq: NP_418258
• UniProt ID: P03018