Recombinant Full Length Atp Synthase Subunit B(Atpf) Protein, His-Tagged

• Cat.No.: RFL33556EF
• Product Overview: Recombinant Full Length ATP synthase subunit b(atpF) Protein (P0ABA1) (1-156aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length (1-156)
• AA Sequence: MNLNATILGQAIAFVLFVLFCMKYV WPPLMAAIEKRQKEIADGLASAERA HKDLDLAKAS ATDQLKKAKAEAQVIIEQANKRRSQ ILDEAKAEAEQERTKIVAQAQAEIE AERKRAREEL RKQVAILAVAGAEKIIERSVDEAAN SDIVDKLVAEL
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: SDS-PAGE
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: atpF
• Synonyms: atpF; c4664; ATP synthase subunit b; ATP synthase F(0 sector subunit b; ATPase subunit I; F-type ATPase subunit b; F-ATPase subunit b
• UniProt ID: P0ABA1

Gene Information

• Gene Name: atpF
• Synonyms: atpF; c4664; ATP synthase subunit b; ATP synthase F(0 sector subunit b; ATPase subunit I; F-type ATPase subunit b; F-ATPase subunit b
• UniProt ID: P0ABA1

Reviews

02/23/2022

The catalytic activity of atpF is excellent, and it can efficiently promote the progress of chemical reactions, and its catalytic effect is impressive.

01/26/2022

The production process of atpF is very environmentally friendly. By optimizing the production process and reducing energy consumption, low carbon emission and energy saving are achieved.

03/23/2021

atpF is highly reproducible, making it ideal for use in experiments or applications that require a large number of replicates.

Q&As

What diseases or symptoms may be caused by atpF gene mutations?

atpF gene mutations may lead to mitochondrial dysfunction, and then cause a series of mitochondrial diseases, such as mitochondrial myopathy and energy metabolism disorders.

What is the function of atpF gene in cells?

The protein encoded by the atpF gene is part of the F0 part of the ATP synthesis complex within the mitochondria, which is involved in the process of converting the proton gradient into ATP energy, thus providing the energy required by the cell.

Is the atpF gene related to a gene or protein?

The atpF gene is closely related to other genes and proteins in the mitochondrial respiratory chain, working in concert with them to accomplish ATP production.

What is the medical or biological importance of atpF gene research?

The study of atpF gene contributes to our deeper understanding of energy production processes in cells, which is important for understanding the mechanisms of mitochondrial diseases and energy metabolism disorders and for developing related therapies.

What is the expression pattern of the atpF gene?

atpF genes are expressed in various tissues and cell types because mitochondria are widely distributed in cells and are required to generate ATP energy in different tissues.

How was the atpF gene discovered?

The atpF gene was discovered through genomics research and advances in molecular biology techniques, and scientists have identified and understood its existence and role through the study of mitochondria in cells.