Recombinant Full Length Danio Rerio Acyl-Coa-Binding Domain-Containing Protein 5-B(Acbd5B) Protein, His-Tagged

• Cat.No.: RFL31399DF
• Product Overview: Recombinant Full Length Danio rerio Acyl-CoA-binding domain-containing protein 5-B(acbd5b) Protein (A5WV69) (1-410aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Danio rerio (Zebrafish) (Brachydanio rerio)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-410)
• AA Sequence: MILRGMEDSKSAQKRFEAAVKVIRS LPEDGSYDLSDDMLVLFYSYYKQAT EGPCNTLKPN SWDPIGKAKWEAWKDLGNMSKDQAM TEYVQEIQLIIETLPVTDRMAELLD ALDPFYEIVE DDDDDDDEGVSKAAPLFTGSTNADK DAERDEEVESESKEGNLDDYMELVE KQKDLSSTTG EKGSLLVFNRSEENSISSLTDGTHS SLNTVDDEEELVYDGSDDEMDSDSM DKPATPEKGS GVRSVRLADGSVVGANMQHGGNREP QCGSQDGKPQGLISPVPHPPTLGTV RNDRISACSG RERGCQGDGGQRGETADRMDKQAIN TQITTILSELEDNMQDVLRRLTTLE QLTASQAEIS PSKTWHSDKPKKRLSWWPLNSSPFT AVLTVLWPFAVHWLVQFYLQRRRRR
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: acbd5b
• Synonyms: acbd5b; si:ch211-112d19.6; Acyl-CoA-binding domain-containing protein 5-B
• UniProt ID: A5WV69

Gene Information

• Gene Name: acbd5b
• Synonyms: acbd5b; si:ch211-112d19.6; Acyl-CoA-binding domain-containing protein 5-B
• UniProt ID: A5WV69

Reviews

12/12/2022

I am impressed by the excellent technical support provided by Creative BioMart for their recombinant ACBD5B protein. They helped me customize the protein to meet my specific research needs.

12/12/2022

We used the Recombinant Zebrafish ACBD5B as a positive control for our experiments, and it worked perfectly.

08/22/2022

I was initially hesitant to purchase recombinant ACBD5B from Creative BioMart, but their excellent customer service and technical support convinced me to give it a try. I'm glad I did because the product exceeded my expectations.

Q&As

Can you produce the ACBD5B fusion protein with MBP tag?

Sure, Creative BioMart supports custom protein production services.

Does the recombinant ACBD5B protein require any special buffer for activity?

The recombinant ACBD5B protein is provided in a buffer optimized for protein stability and activity. However, depending on the specific application, it may require a different buffer or conditions for optimal activity.

I am a first-time buyer, can I get a discount on recombinant ACBD5B protein?

Coupons are offered to all first-time customers. We extend our gratitude for your inquiry and eagerly anticipate your subsequent order.

Does your recombinant ACBD5B protein have a fusion protein with a tag other than the His tag?

Certainly, we can help you to select the appropriate expression system and tag in response to your specific requirements.

Are there any known interactions of the ACBD5B protein with other proteins or molecules?

The ACBD5B protein has been shown to interact with various proteins and molecules involved in lipid metabolism, such as peroxisomal membrane protein PEX19 and arachidonic acid. These interactions play a crucial role in regulating lipid metabolism and transport in cells.

Can the recombinant ACBD5B protein be used in functional assays?

Yes, it has been shown to be functional in lipid binding assays and has been used in various in vitro experiments to investigate its role in lipid metabolism.

Can your ACBD5B proteins be used for western blotting?

Yes, our recombinant ACBD5B protein displays immense versatility and can be employed for various applications by exploiting the antigen-antibody reactivity.

What is the endotoxin level of the recombinant ACBD5B protein?

The endotoxin level of the recombinant ACBD5B protein offered by Creative BioMart can be less than 0.1 eu/μg, as determined by LAL assay. In addition, you can choose the concentration you need when submitting the inquiry form.

What downstream applications are your ACBD5B proteins suitable for?

It is suitable for a variety of applications such as ELISA, western blotting, antibody production, and protein array.

Can the recombinant ACBD5B protein be used in cell culture experiments?

Yes, it can be used in cell culture experiments to investigate its role in lipid metabolism and transport. However, it is important to note that the protein may contain bacterial endotoxins, which could cause an immune response in cells. And you can choose the low endotoxin concentration when submitting the inquiry form.