Recombinant Full Length Danio Rerio Acyl-Coa-Binding Domain-Containing Protein 5A(Acbd5A) Protein, His-Tagged

• Cat.No.: RFL1625DF
• Product Overview: Recombinant Full Length Danio rerio Acyl-CoA-binding domain-containing protein 5A(acbd5a) Protein (Q502L1) (1-501aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Danio rerio (Zebrafish) (Brachydanio rerio)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-501)
• AA Sequence: MEGDSNPLYEQRFNAAVKVIQNLPP NGSFQPSHDMMLKFYSYYKQATQGP CNIPRPGFWD PVGKAKWDAWSSLGEMPKEEAMAAY VDDLKLILESMPVSSEVEELLQVIG PFYELVDEKR KITQVSDLSTGFGNLLSSPPKCVTK SIIRTMEMNGNLEGYPIKTAETLKV KSIDLEDRED DDDEDEEGERDEVEEFKEVEKASQP KKRVSAGRPKGPVSNGSISQHKGLS NGTHGSKSDL NRQESEENTEHMNHDGGIVELNGHL NSEKDKEEDVSSSHHVASDSDSEVY CDSVDQFGGE DGSEIHMNRSLEVLEESHSTPSSTG DIRSQDDELLGREEGVQHGGEDGRG SRGGAQRREL PVKRSDSSVVRRGRGSRSPASGSGS AGPQQGSGGDGERWGADGPMTENLN EQIICALARL QDDMQSVLQRLHTLEALTASQARSL ALPSDYLTTPANRNKKKPSWWPFDV SLGTVAFAVV WPFVVQWLIRVYVQRRRRRIN
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: acbd5a
• Synonyms: acbd5a; acbd5; zgc:112043; Acyl-CoA-binding domain-containing protein 5A
• UniProt ID: Q502L1

Gene Information

• Gene Name: acbd5a
• Synonyms: acbd5a; acbd5; zgc:112043; Acyl-CoA-binding domain-containing protein 5A
• UniProt ID: Q502L1

Reviews

09/18/2021

I am very happy with the recombinant Zebrafish ACBD5A from Creative BioMart. It was exactly what we needed for our experiments.

08/10/2021

The recombinant ACBD5A protein is of great quality and purity, making it ideal for our assays based on antigen-antibody reactions.

10/12/2020

The purity and quality of the Recombinant Zebrafish ACBD5A exceeded my expectations. I will definitely purchase it again.

Q&As

What are the potential safety concerns of using recombinant ACBD5A protein?

There are no known safety concerns or side effects associated with using recombinant ACBD5A protein for research purposes. However, as with any protein or biological material, it is important to handle and dispose of the protein properly to minimize the risk of contamination or exposure. It is also important to follow the safety guidelines and regulations of your institution or country regarding the use of recombinant proteins.

What are the downstream assays for recombinant ACBD5A protein?

It can be used for various applications in cell biology, biochemistry, and drug discovery research, such as protein-protein interaction studies, lipid metabolism assays, and membrane trafficking assays. It can also be used as an antigen in antibody production or as a standard in ELISA or Western blot assays. Downstream assays may include fluorescence resonance energy transfer (FRET), enzyme-linked immunosorbent assay (ELISA), or surface plasmon resonance (SPR).

How to assess the purity and quality of recombinant ACBD5A protein?

The protein is typically assessed for purity by SDS-PAGE or HPLC analysis and for quality by biophysical methods such as circular dichroism spectroscopy, dynamic light scattering, or mass spectrometry.

Can I use recombinant ACBD5A protein to prepare specific antibodies for immunoblotting?

Yes, and we recommend that you select the conserved motif of the ACBD5A of the corresponding species.

What is the recommended buffer for reconstituting ACBD5A protein?

It may depend on the specific product. In general, the protein should be reconstituted in a buffer compatible with the downstream assay or application. The buffer may contain stabilizing agents, salts, or detergents to maintain the protein stability and activity.

I am going to order recombinant zebrafish ACBD5A (ACBD5A-2819Z), can I get a discount?

If you are shopping on our site for the first time, you can apply for a first-time purchase coupon. In addition, we will offer promotions during holidays. For specific discounts, please contact our customer service staff.

There is no ACBD5A protein I need in the product list, can it be customized?

Yes, we support custom protein production services.

I plan to order ACBD5A protein, is there any discount for bulk?

We can offer a bulk quote for each product available on our website. For specific prices, please contact our sales representatives.

Can you guarantee low endotoxin levels in the recombinant ACBD5A protein?

Creative BioMart unequivocally assures the elevated caliber of our products. If your research requires more stringent specifications for protein endotoxin levels, then we kindly recommend opting for a lower parameter during the submission of your inquiry form.

Does the His tag of Recombinant Zebrafish ACBD5A affect the activity of the protein?

The His tag is used for affinity purification, it may or may not affect protein activity. If you are concerned that the tag may interfere with protein activity, we can also provide a custom tag-free ACBD5A protein production service.