Recombinant Full Length Dictyostelium Discoideum Abc Transporter B Family Member 4(Abcb4) Protein, His-Tagged

• Cat.No.: RFL26679DF
• Product Overview: Recombinant Full Length Dictyostelium discoideum ABC transporter B family member 4(abcB4) Protein (Q54W24) (1-767aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Dictyostelium discoideum (Slime mold)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-767)
• AA Sequence: MNSLLRCCLIKPINIKNTTSFINKL NPKSIIQKSNNYNNINKNILNKFKD SSSNYFINSN KILEICNNNNNNNKYNVNKRNYSSS SNSGNNNNNNYNNKNNNNNNESFYK KFENSFEIKR IKDILFIIVGTFVIFKIYDNKIGIV FCENEIDKETILNDPYDKIPNEIIV ELKDSAKDDN IIEESDEDDGIKKISSFKLFFKTIG NDIWLFGFGIITAFFSSWVGLQIPK VFGVLIDCTK NGDSLQGPAIQAIFILLAQAGLNFL YSTMISVACERYSARLRSTLFGAML EQEIGFFDQN STGDLINRLSSDVQLVRSALKHSVS LGVKSFGQIVGGVISLILISPKLSL GMMTILPTMV SVGTFYAGWLKSLSVRSQRAQAQST IVAEEAIGNIRTVQAFSNQHYESER FIEKNQHSLA LSTESGVQIGIFQGVTSLALNSVSL LVYWYGGTLVSRGEMTGGQLTSFII HTMNMQSSFS QLSILFTQIMSAMGGMQRITELINR VPLINSNQGFKLRELKGEIKFINVD FKYPTRPHVH VLNGLNLTLKPGQVVALAGSSGGGK STIAGLLERFYDISNGDITIDGYSI KQLNAKWLRS RIGIVSQEPSLFATTILENLRYGNP NATEDEIIEAAKLANAHQFISNFPK GYETIVGERG VQLSGGQKQRIAIARAILKNPQIII LDEATSALDSQSELLVQTALDNLMK GRTTLVIAHR LSTVQNADLIGVLSHGKIAEFGNHN ELMNHKGLYYKLVQRQLSQQQQ
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: abcB4
• Synonyms: abcB4; DDB_G0279915; ABC transporter B family member 4; ABC transporter ABCB.4
• UniProt ID: Q54W24

Gene Information

• Gene Name: abcB4
• Synonyms: abcB4; DDB_G0279915; ABC transporter B family member 4; ABC transporter ABCB.4
• UniProt ID: Q54W24

Reviews

12/07/2022

I purchased this protein product for Western blotting, and the feedback results are not bad, and it can meet my experimental needs.

06/11/2019

I have received my customized recombinant ABCB4 protein, and the delivery cycle is short

Q&As

Do you offer low endotoxin recombinant ABCB4 protein?

The endotoxin level of recombinant protein can be customized according to customer needs, and you can communicate with our technical experts in detail.

Is the tag of the recombinant ABCB4 protein at the N-terminus or the C-terminus?

We provide these two types of recombinant proteins, you can buy or customize according to your needs.

The purity of ABCB4-1091M is >80%, can I order ABCB4 protein with higher purity?

We provide recombinant protein products with different purities, and you can communicate with our technical experts about your customized needs.

How to choose the two product forms of ABCB4-397R?

Both have their own advantages, and the freeze-dried form is more stable than the liquid form, but the freeze-drying process may cause certain damage to the structure and activity of the protein.

ABCB4 protein is expressed in hepatocytes, is it related to the formation of bile?

Bile contains three main lipids: cholesterol, phospholipids and bile acids. ABCB4 protein participates in phospholipid transport and is crucial for the formation of bile.

Is the ABCB4 protein the same as MDR3?

ABCB4 protein is also known as multidrug resistance protein 3 (MDR3).

How long should I incubate with the primary antibody at room temperature when performing a western blot for ABCB4?

It is generally recommended to incubate at room temperature for about two hours, but the exact time may also vary.

How should I quantify the results when I perform a western blot of ABCB4 protein?

Quantification can be performed in the linear range of detection using ImageJ software.

The ABCB4 protein (Cat.No. : ABCB4-397R) has two product forms, can I specify the lyophilized powder product to be shipped?

If you have requirements for the product format, please note when placing an order.

I want to use ABCB4 protein for Western blotting, how should I determine the concentration of proteins in the experiment?

You can use the Uptima bicinchoninic acid protein assay to determine the protein concentrations.