Recombinant Full Length Human ACAT1 Protein, GST-tagged
Cat.No. : | ACAT1-913HF |
Product Overview : | Human ACAT1 full-length ORF ( AAH10942, 16 a.a. - 162 a.a.) recombinant protein with GST-tag at N-terminal. |
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Description : | This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009] |
Source : | In Vitro Cell Free System |
Species : | Human |
Tag : | GST |
Molecular Mass : | 95.7 kDa |
Protein Length : | 162 amino acids |
AA Sequence : | MSGCGLFLRT TAAARACRGL VVSTANRRLL RTSPPVRAFA KELFLGKIKK KEVFPFPEVS QDELNEINQF LGPVEKFFTE EVDSRKIDQE GKIPDETLEK LKSLGLFGLQ VPEEYGGLGF SNTMYSRLGE IISMDGSITV TLAAHQAIGL KGIILAGTEE QKAKYLPKLA SGEHIAAFCL TEPASGSDAA SIRSRATLSE DKKHYILNGS KVWITNGGLA NIFTVFAKTE VVDSDGSVKD KITAFIVERD FGGVTNGKPE DKLGIRGSNT CEVHFENTKI PVENILGEVG DGFKVAMNIL NSGRFSMGSV VAGLLKRLIE MTAEYACTRK QFNKRLSEFG LIQEKFALMA QKAYVMESMT YLTAGMLDQP GFPDCSIEAA MVKVFSSEAA WQCVSEALQI LGGLGYTRDY PYERILRDTR ILLIFEGTNE ILRMYIALTG LQHAGRILTT RIHELKQAKV STVMDTVGRR LRDSLGRTVD LGLTGNHGVV HPSLADSANK FEENTYCFGR TVETLLLRFG KTIMEEQLVL KRVANILINL YGMTAVLSRA SRSIRIGLRN HDHEVLLANT FCVEAYLQNL FSLSQLDKYA PENLDEQIKK VSQQILEKRA YICAHPLDRT C |
Applications : | Enzyme-linked Immunoabsorbent Assay Western Blot (Recombinant protein) Antibody Production Protein Array |
Storage : | Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
Storage Buffer : | 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
Gene Name : | ACAT1 acetyl-CoA acetyltransferase 1 [ Homo sapiens ] |
Official Symbol : | ACAT1 |
Synonyms : | ACAT1; acetyl-CoA acetyltransferase 1; ACAT, acetyl Coenzyme A acetyltransferase 1; acetyl-CoA acetyltransferase, mitochondrial; acetoacetyl Coenzyme A thiolase; THIL; acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase; T2; MAT; ACAT |
Gene ID : | 38 |
mRNA Refseq : | NM_000019 |
Protein Refseq : | NP_000010 |
MIM : | 607809 |
UniProt ID : | P24752 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Studies have demonstrated that upregulation of ACAT1 expression and activity can lead to the development of hepatic steatosis, a hallmark of NAFLD. Inhibiting ACAT1 has been proposed as a potential therapeutic approach for treating NAFLD.
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ACAT1 is a key enzyme involved in the biosynthesis of cholesterol and ketone bodies, as well as in the breakdown of fatty acids through beta-oxidation. It plays a critical role in maintaining lipid homeostasis in the liver and regulating the levels of circulating cholesterol and fatty acids.
ACAT1 expression is regulated by a variety of factors, including hormonal signals such as insulin and glucagon, as well as by the levels of intracellular metabolites such as acetyl-CoA and cholesterol. In addition, ACAT1 activity is subject to post-translational modifications such as phosphorylation, which can modulate its enzymatic activity.
ACAT1 is a homotetrameric protein that consists of four identical subunits. Each subunit contains a catalytic domain that is responsible for the transfer of acetyl-CoA, as well as a regulatory domain that modulates enzymatic activity. The crystal structure of ACAT1 has been solved, revealing key structural features such as the location of the active site and the binding sites for acetyl-CoA and cholesterol.
Our recombinant ACAT1 protein is primarily used as a research tool and is not approved for diagnostic or therapeutic purposes.
Inhibiting ACAT1 has been proposed as a potential therapeutic approach for treating NAFLD, as well as other metabolic disorders such as hypercholesterolemia and atherosclerosis.
The recombinant ACAT1 protein can be used in a variety of research and application settings, such as studying the biochemistry and enzymology of the ACAT1 enzyme, screening for inhibitors or activators of the enzyme, or developing diagnostic assays for diseases related to cholesterol metabolism. The protein can also be used as a reference standard or control in analytical methods such as mass spectrometry or ELISA. However, it is important to note that the specific use of the recombinant ACAT1 protein will depend on the goals and requirements of the individual research or application.
Recombinant protein is typically quantified using a combination of techniques, such as UV spectrophotometry, Bradford protein assay, or bicinchoninic acid (BCA) assay. To characterize the protein, a variety of methods can be used, including SDS-PAGE, Western blotting, mass spectrometry, and enzyme activity assays.
Customer Reviews (3)
Write a reviewThe recombinant human ACAT1 protein was affordable and highly effective.
The customization services provided by Creative BioMart's technical support team for the recombinant ACAT1 protein were invaluable. I am grateful for their expertise and professionalism.
I appreciate the level of expertise and professionalism demonstrated by Creative BioMart's scientists. Their Recombinant Human ACAT1 Protein helped me achieve the results I needed for my project.
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