Recombinant Full Length Human ACOX3 Protein, GST-tagged

• Cat.No.: ACOX3-799HF
• Product Overview: Human ACOX3 full-length ORF (AAH17053.1, 1 a.a. - 624 a.a.) recombinant protein with GST tag at N-terminal.

Specification

• Description: Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3)is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. Unlike the rat homolog, the human gene is expressed in very low amounts in liver such that its mRNA was undetectable by routine Northern-blot analysis or its product by immunoblotting or by enzyme activity measurements. However the human cDNA encoding a 700 amino acid protein with a peroxisomal targeting C-terminal tripeptide S-K-L was isolated and is thought to be expressed under special conditions such as specific developmental stages or in a tissue specific manner in tissues that have not yet been examined. [provided by RefSeq, Jul 2008]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 96 kDa
• Protein Length: 624 amino acids
• AA Sequence: MASTVEGGDT ALLPEFPRGP LDAYRARASF SWKELALFTE GEGMLRFKKT IFSALENDPL FARSPGADLS LEKYRELNFL RCKRIFEYDF LSVEDMFKSP LKVPALIQCL GMYDSSLAAK YLLHSLVFGS AVYSSGSERH LTYIQKIFRM EIFGCFALTE LSHGSNTKAI RTTAHYDPAT EEFIIHSPDF EAAKFWVGNM GKTATHAVVF AKLCVPGDQC HGLHPFIVQI RDPKTLLPMP GVMVGDIGKK LGQNGLDNGF AMFHKVRVPR QSLLNRMGDV TPEGTYVSPF KDVRQRFGAS LGSLSSGRVS IVSLAILNLK LAVAIALRFS ATRRQFGPTE EEEIPVLEYP MQQWRLLPYL AAVYALDHFS KSLFLDLVEL QRGLASGDRS ARQAELGREI HALASASKPL ASWTTQQGIQ ECREACGGHG YLAMNRLGVL RDDNDPNCTY EGDNNILLQQ TSNYLLGLLA HQVHDGACFR SPLKSVDFLD AYPGILDQKF EVSSVADCLD SAVALAAYKW LVCYLLRETY QKLNQEKRSG SSDFEARNKC QVSHGRPLAL AFVELTVVQR FHEHVHQPSV PPSLRAVLGR LSALYALWSL SRHAALLYRA ERRCSCPGRR DRSS
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ACOX3 acyl-CoA oxidase 3, pristanoyl [ Homo sapiens ]
• Official Symbol: ACOX3
• Synonyms: ACOX3; acyl-CoA oxidase 3, pristanoyl; acyl Coenzyme A oxidase 3, pristanoyl; peroxisomal acyl-coenzyme A oxidase 3; BRCACox; pristanoyl-CoA oxidase; branched-chain acyl-CoA oxidase; acyl-Coenzyme A oxidase 3, pristanoyl
• Gene ID: 8310
• mRNA Refseq: NM_001101667
• Protein Refseq: NP_001095137
• MIM: 603402
• UniProt ID: O15254

Q&As

Can ACOX3 protein be used as a biomarker for metabolic disorders?

ACOX3 protein levels and activity have been shown to be altered in various metabolic disorders, such as X-ALD and non-alcoholic fatty liver disease (NAFLD), suggesting that it could be a potential biomarker for these conditions.

Are there any drugs that target ACOX3 protein?

There are currently no drugs that specifically target ACOX3, but certain drugs used to treat metabolic disorders, such as bezafibrate and gemfibrozil, have been shown to stimulate the activity of ACOX3 and other fatty acid oxidation enzymes.

Are there any genetic mutations associated with ACOX3 protein?

Mutations in the ACOX3 gene have been associated with certain peroxisomal disorders, such as X-linked adrenoleukodystrophy (X-ALD). These mutations can result in a decrease in ACOX3 activity, leading to the accumulation of VLCFAs and other metabolites that contribute to disease pathology.

Can ACOX3 be used in biotechnology applications?

ACOX3 has been used in biotechnology applications such as the production of long-chain fatty alcohols and alkanes for use as biofuels. Expression of ACOX3 can increase the production of these compounds in microbes such as E. coli.

How is ACOX3 protein regulated in cells?

ACOX3 expression and activity are regulated by a variety of factors, including peroxisome proliferator-activated receptor (PPAR) alpha, which is a key regulator of fatty acid oxidation. ACOX3 expression is also influenced by various signaling pathways and transcription factors, such as the sterol regulatory element-binding protein (SREBP) pathway.

Can ACOX3 protein be used to improve athletic performance?

There is limited research on the potential use of ACOX3 protein to improve athletic performance. While ACOX3 is involved in fatty acid oxidation, which is a major energy source for endurance exercise, further studies are needed to determine its potential as a performance-enhancing substance.

Are there diagnostic applications for ACOX3 protein?

ACOX3 protein levels have been shown to be altered in certain diseases such as peroxisomal disorders, suggesting its potential as a diagnostic marker for these conditions. However, further studies are needed to determine the diagnostic utility of ACOX3 in clinical settings.

Can ACOX3 protein be used as a therapeutic target?

ACOX3 has been identified as a potential therapeutic target for metabolic disorders, particularly X-ALD. However, further research is needed to develop targeted therapies.

How does ACOX3 protein interact with other enzymes involved in fatty acid metabolism?

ACOX3 works in concert with other enzymes involved in fatty acid metabolism, including carnitine palmitoyltransferase (CPT) and acyl-CoA dehydrogenase (ACAD). CPT transfers fatty acids into the mitochondria for oxidation, while ACAD is responsible for the initial step in beta-oxidation.

Are there any dietary interventions that can increase ACOX3 activity?

Certain dietary interventions, such as fasting and the consumption of medium-chain triglycerides (MCTs), have been shown to increase ACOX3 activity and fatty acid oxidation in the liver.

Is ACOX3 protein involved in other physiological processes?

In addition to its role in the oxidation of VLCFAs, ACOX3 has also been shown to play a role in the synthesis of certain lipids, such as sphingolipids and plasmalogens. These lipids have important functions in cellular membranes and signaling pathways.

Is ACOX3 involved in cancer development?

The role of ACOX3 in cancer development is not well understood. Some studies have suggested that ACOX3 expression is decreased in certain types of cancer, such as bladder cancer, while others have shown increased expression in breast cancer. More research is needed to determine the potential role of ACOX3 in cancer development and progression.

Can ACOX3 be used as a target for drug development?

ACOX3 has been suggested as a potential target for drug development in a variety of metabolic disorders, including peroxisomal disorders and nonalcoholic fatty liver disease (NAFLD). Inhibition of ACOX3 activity has been shown to reduce the accumulation of VLCFA, which can contribute to disease pathology.

Could ACOX3 be used as a biomarker for metabolic disorders?

ACOX3 expression has been shown to be altered in certain metabolic disorders such as peroxisomal disorders and NAFLD, suggesting its potential as a biomarker for these conditions. However, further studies are needed to determine its diagnostic utility in clinical settings.

What techniques are used to study ACOX3 protein?

Various techniques are used to study ACOX3 protein, including Western blotting, immunohistochemistry, and mass spectrometry. In addition, genetic manipulation of cells and animal models can be used to investigate the physiological functions and regulation of ACOX3.