Recombinant Full Length Human ACTL7A Protein, GST-tagged

• Cat.No.: ACTL7A-847HF
• Product Overview: Human ACTL7A full-length ORF ( NP_006678.1, 1 a.a. - 435 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene (ACTL7A), and related gene, ACTL7B, are intronless, and are located approximately 4 kb apart in a head-to-head orientation within the familial dysautonomia candidate region on 9q31. Based on mutational analysis of the ACTL7A gene in patients with this disorder, it was concluded that it is unlikely to be involved in the pathogenesis of dysautonomia. The ACTL7A gene is expressed in a wide variety of adult tissues, however, its exact function is not known. [provided by RefSeq, Jul 2008]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 75 kDa
• Protein Length: 435 amino acids
• AA Sequence: MWAPPAAIMG DGPTKKVGNQ APLQTQALQT ASLRDGPAKR AVWVRHTSSE PQEPTESKAA KERPKQEVTK AVVVDLGTGY CKCGFAGLPR PTHKISTTVG KPYMETAKTG DNRKETFVGQ ELNNTNVHLK LVNPLRHGII VDWDTVQDIW EYLFRQEMKI APEEHAVLVS DPPLSPHTNR EKYAEMLFEA FNTPAMHIAY QSRLSMYSYG RTSGLVVEVG HGVSYVVPIY EGYPLPSITG RLDYAGSDLT AYLLGLLNSA GNEFTQDQMG IVEDIKKKCC FVALDPIEEK KVPLSEHTIR YVLPDGKEIQ LCQERFLCSE MFFKPSLIKS MQLGLHTQTV SCLNKCDIAL KRDLMGNILL CGGSTMLSGF PNRLQKELSS MCPNDTPQVN VLPERDSAVW TGGSILASLQ GFQPLWVHRF EYEEHGPFFL YRRCF
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ACTL7A actin-like 7A [ Homo sapiens ]
• Official Symbol: ACTL7A
• Synonyms: ACTL7A; actin-like 7A; actin-like protein 7A; actin-like 7-alpha; actin-like-7-alpha
• Gene ID: 10881
• mRNA Refseq: NM_006687
• Protein Refseq: NP_006678
• MIM: 604303
• UniProt ID: Q9Y615

Reviews

02/05/2020

The company's customer support team is highly knowledgeable and responsive, readily available to address any technical or product-related queries.

03/13/2019

In our experiments, the protein product consistently exhibits robust and dose-dependent effects, leading to significant changes in the desired cellular responses.

01/22/2019

Each batch of the protein product shows minimal batch-to-batch variation, providing consistent results and reducing experimental variability.

04/29/2018

The protein product is manufactured using high-quality raw materials sourced from reputable suppliers, ensuring its purity and reliability.

02/16/2017

The protein product is carefully packaged using appropriate storage conditions, such as freeze-drying or cold chain shipping, to maintain its stability and integrity.

Q&As

How might the altered localization of ZPBP in Actl7a KO mice affect sperm-zona pellucida binding ability?

The altered localization of ZPBP in Actl7a KO mice suggests a potential impact on sperm-zona pellucida binding ability.

How does Actl7a deficiency contribute to male infertility?

Actl7a deficiency affects the localization of ZPBP, potentially impacting sperm-zona pellucida binding. It also leads to abnormalities in PLCZ1 localization and expression, resulting in reduced calcium oscillations in oocytes.

Where is ACTL7A found in the human body?

ACTL7A is found in various tissues, including the brain, heart, liver, and skeletal muscle.

Can ACTL7A expression be regulated?

Yes, ACTL7A expression can be regulated by various factors, including signaling pathways and transcription factors.

Does ACTL7A have any known paralogs or similar genes?

Yes, ACTL7B is a paralog of ACTL7A, sharing sequence similarities and similar functions in chromatin remodeling.

What is the molecular function of ACTL7A?

ACTL7A is involved in nucleosome assembly, histone binding, and ATP-dependent chromatin remodeling.

What do findings suggest about Actl7a and male infertility?

It revearls the importance of ACTL7A in the complex processes involved in fertilization.

What condition is associated with Actl7a deficiency?

Actl7a deficiency is linked to male infertility.

What is the consequence of the abnormal localization and expression of PLCZ1?

The abnormal localization and expression of PLCZ1 resulted in reduced calcium oscillations in oocytes.

What are the known interactions of ACTL7A?

ACTL7A interacts with other proteins involved in chromatin remodeling, such as SMARCA4 and SMARCA2.

Is there any research on the role of ACTL7A in neurodevelopmental disorders?

Yes, studies have shown that ACTL7A mutations can contribute to neurodevelopmental disorders, and further research is being conducted in this area.

Is ACTL7A associated with cancer?

There is currently no significant evidence linking ACTL7A to cancer development or progression.

What was found regarding the localization of ZPBP in Actl7a homozygous KO male mice?

The localization of ZPBP was found to be altered in the sperm of Actl7a homozygous KO male mice.

Can ACTL7A be a potential therapeutic target?

While ACTL7A itself is not currently considered a therapeutic target, understanding its role in chromatin remodeling could have implications for future therapeutic interventions.

Are there any genetic disorders associated with ACTL7A mutations?

Yes, mutations in ACTL7A have been linked to developmental disorders, including intellectual disability and autism spectrum disorder.

What is the relationship between ACTL7A and ZPBP?

ACTL7A and ZPBP can form a complex that is potentially involved in acrosomal formation.

What will happen if ACTL7A undergoes a mutation?

Mutations in the Actl7a gene have been reported to lead to male infertility

What is ACTL7A?

ACTL7A plays a role in chromatin remodeling and gene transcription regulation.

What abnormalities were observed in the localization and expression of the PLCZ1 protein?

Further studies revealed that the localization and expression of the PLCZ1 protein were abnormal in misshapen sperm.

Is ACTL7A involved in any specific biological processes?

Yes, ACTL7A is involved in processes such as transcriptional regulation, DNA repair, and neuronal development.