Recombinant Full Length Human ALPK1 Protein, C-Flag-tagged

• Cat.No.: ALPK1-394HFL
• Product Overview: Recombinant Full Length Human ALPK1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 138.7 kDa
• AA Sequence: MNNQKVVAVLLQECKQVLDQLLLEA PDVSEEDKSEDQRCRALLPSELRTL IQEAKEMKWPFVPEKWQYK QAVGPEDKTNLKDVIGAGLQQLLAS LRASILARDCAAAAAIVFLVDRFLY GLDVSGKLLQVAKGLHKLQ PATPIAPQVVIRQARISVNSGKLLK AEYILSSLISNNGATGTWLYRNESD KVLVQSVCIQIRGQILQKL GMWYEAAELIWASIVGYLALPQPDK KGLSTSLGILADIFVSMSKNDYEKF KNNPQINLSLLKEFDHHLL SAAEACKLAAAFSAYTPLFVLTAVN IRGTCLLSYSSSNDCPPELKNLHLC EAKEAFEIGLLTKRDDEPV TGKQELHSFVKAAFGLTTVHRRLHG ETGTVHAASQLCKEAMGKLYNFSTS SRSQDREALSQEVMSVIAQ VKEHLQVQSFSNVDDRSYVPESFEC RLDKLILHGQGDFQKILDTYSQHHT SVCEVFESDCGNNKNEQKD AKTGVCITALKTEIKNIDTVSTTQE KPHCQRDTGISSSLMGKNVQRELRR GGRRNWTHSDAFRVSLDQD VETETEPSDYSNGEGAVFNKSLSGS QTSSAWSNLSGFSSSASWEEVNYHV DDRSARKEPGKEHLVDTQC STALSEELENDREGRAMHSLHSQLH DLSLQEPNNDNLEPSQNQPQQQMPL TPFSPHNTPGIFLAPGAGL LEGAPEGIQEVRNMGPRNTSAHSRP SYRSASWSSDSGRPKNMGTHPSVQK EEAFEIIVEFPETNCDVKD RQGKEQGEEISERGAGPTFKASPSW VDPEGETAESTEDAPLDFHRVLHNS LGNISMLPCSSFTPNWPVQ NPDSRKSGGPVAEQGIDPDASTVDE EGQLLDSMDVPCTNGHGSHRLCILR QPPGQRAETPNSSVSGNIL FPVLSEDCTTTEEGNQPGNMLNCSQ NSSSSSVWWLKSPAFSSGSSEGDSP WSYLNSSGSSWVSLPGKMR KEILEARTLQPDDFEKLLAGVRHDW LFQRLENTGVFKPSQLHRAHSALLL KYSKKSELWTAQETIVYLG DYLTVKKKGRQRNAFWVHHLHQEEI LGRYVGKDYKEQKGLWHHFTDVERQ MTAQHYVTEFNKRLYEQNI PTQIFYIPSTILLILEDKTIKGCIS VEPYILGEFVKLSNNTKVVKTEYKA TEYGLAYGHFSYEFSNHRD VVVDLQGWVTGNGKGLIYLTDPQIH SVDQKVFTTNFGKRGIFYFFNNQHV ECNEICHRLSLTRPSMEKP
  CTTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase

Gene Information

• Gene Name: ALPK1 alpha kinase 1 [ Homo sapiens (human) ]
• Official Symbol: ALPK1
• Synonyms: LAK; ROSAH; 8430410J10Rik
• Gene ID: 80216
• mRNA Refseq: NM_025144.4
• Protein Refseq: NP_079420.3
• MIM: 607347
• UniProt ID: Q96QP1

Reviews

03/26/2022

Highly effective fusion protein detection.

06/16/2019

Exceptional protein-protein interaction analysis.

02/09/2017

Specific target detection in immunohistochemistry.

Q&As

Can ALPK1 kinase activity be modulated by specific small molecules or inhibitors?

Yes, ALPK1 kinase activity can be modulated by specific small molecule inhibitors that target its ATP-binding site.

How does ALPK1 influence intracellular signaling cascades or gene expression patterns?

ALPK1 influences intracellular signaling cascades such as MAPK and AKT pathways and can modulate the expression of specific target genes.

What is the impact of ALPK1 knockout or overexpression on cellular phenotypes?

ALPK1 knockout or overexpression can lead to altered cell morphology, proliferation rates, and cell cycle progression.

What is the subcellular localization of ALPK1 in human cells?

ALPK1 is primarily localized in the cytoplasm of human cells.

Is ALPK1 involved in the regulation of cellular apoptosis or survival pathways?

ALPK1 has been associated with both pro-apoptotic and anti-apoptotic signaling pathways, suggesting a complex role in cellular survival.

Does ALPK1 expression change under specific cellular stress conditions?

Yes, the expression of ALPK1 can be upregulated or downregulated in response to various stress stimuli.

How does ALPK1 contribute to cellular proliferation and growth?

ALPK1 is implicated in promoting cellular proliferation and growth.

Does ALPK1 play a role in cellular migration or invasion processes?

ALPK1 has been implicated in regulating cellular migration and invasion in certain cell types.

Are there any specific post-translational modifications that regulate ALPK1 activity?

Phosphorylation is a known post-translational modification that can regulate ALPK1 activity.

Are there any known interacting proteins or signaling pathways associated with ALPK1?

Several interacting proteins and signaling pathways have been identified, including protein kinases, phosphatases, and cytoskeletal regulators.