Recombinant Full Length Human ARIH2 Protein, C-Flag-tagged

• Cat.No.: ARIH2-1192HFL
• Product Overview: Recombinant Full Length Human ARIH2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have been found for this gene, although the full-length nature of some of them have not been determined yet.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 57.6 kDa
• AA Sequence: MSVDMNSQGSDSNEEDYDPNCEEEE EEEEDDPGDIEDYYVGVASDVEQQG ADAFDPEEYQFTCLTYKESE GALNEHMTSLASVLKVSHSVAKLIL VNFHWQVSEILDRYKSNSAQLLVEA RVQPNPSKHVPTSHPPHHCA VCMQFVRKENLLSLACQHQFCRSCW EQHCSVLVKDGVGVGVSCMAQDCPL RTPEDFVFPLLPNEELREKY RRYLFRDYVESHYQLQLCPGADCPM VIRVQEPRARRVQCNRCNEVFCFKC RQMYHAPTDCATIRKWLTKC ADDSETANYISAHTKDCPKCNICIE KNGGCNHMQCSKCKHDFCWMCLGDW KTHGSEYYECSRYKENPDIV NQSQQAQAREALKKYLFYFERWENH NKSLQLEAQTYQRIHEKIQERVMNN LGTWIDWQYLQNAAKLLAKC RYTLQYTYPYAYYMESGPRKKLFEY QQAQLEAEIENLSWKVERADSYDRG DLENQMHIAEQRRRTLLKDF
  HDTTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: ARIH2 ariadne RBR E3 ubiquitin protein ligase 2 [ Homo sapiens (human) ]
• Official Symbol: ARIH2
• Synonyms: ARI2; TRIAD1
• Gene ID: 10425
• mRNA Refseq: NM_006321.4
• Protein Refseq: NP_006312.1
• MIM: 605615
• UniProt ID: O95376

Reviews

01/21/2023

This flexibility ensures that the ARIH2 protein can address my specific research objectives efficiently.

08/12/2022

Their experienced team is dedicated to resolving any issues or challenges I may encounter during my experiments, providing prompt and effective solutions.

07/30/2022

the manufacturer's technical expertise can be advantageous to researchers.

02/16/2022

Its purity, stability, and consistency ensure reliable and reproducible results, giving me confidence in the integrity of my research findings.

01/30/2022

This versatility enables researchers to explore ARIH2's function and role in a range of biological processes, making it a valuable tool across diverse research fields.

12/07/2021

With their assistance, I can overcome obstacles and optimize my experimental setup.

01/03/2021

With the ARIH2 protein and the manufacturer's support, I am confident in obtaining accurate and meaningful results for my scientific investigations.

10/27/2020

In addition to the advantages of ARIH2 protein itself, the manufacturer can play a crucial role in supporting the research efforts.

04/16/2020

This quality control is essential for obtaining reliable and reproducible data, as any variability in protein quality can introduce confounding factors into experiments.

12/18/2017

They can offer guidance on experimental design, protocol optimization, and troubleshooting, helping researchers overcome any challenges encountered during their trials.

Q&As

Is there any evidence for the involvement of ARIH2 in cancer development?

Yes, ARIH2 has been implicated in cancer development and progression. Its dysregulation has been observed in several cancer types, and studies have shown its role in regulating the stability and activity of oncoproteins and tumor suppressors.

Are there any known substrates of ARIH2 protein?

ARIH2 has been shown to ubiquitinate various proteins, including NEDD8-conjugating enzyme UbcH7, p53, and the EphA2 receptor, among others.

Are there any known interacting partners of ARIH2?

Yes, ARIH2 interacts with various proteins, including the NEDD8-conjugating enzyme UbcH7, p53, and multiple components of the immune response pathway.

Does ARIH2 have any known disease associations?

Research has suggested that ARIH2 may be involved in the pathogenesis of certain diseases, including cancer and neurodegenerative disorders.

How does the ARIH2 protein carry out ubiquitin ligase activity?

ARIH2 functions as an E3 ubiquitin ligase by binding to the target protein and facilitating the transfer of ubiquitin from the E2 ubiquitin-conjugating enzyme to the target protein.

Does ARIH2 play a role in the immune response?

Yes, ARIH2 has been implicated in the regulation of immune responses. It is involved in the degradation of immune signaling proteins, such as IKKγ and TRAF3, thereby modulating the activation of immune pathways.

What future directions of research are being pursued regarding the ARIH2 protein?

Future research on ARIH2 aims to further elucidate its molecular mechanisms, identify additional substrates, explore its potential as a therapeutic target, and understand its role in various diseases.

What techniques are used to study the ARIH2 protein?

Researchers employ techniques such as gene expression analysis, protein-protein interaction studies, structural biology, and molecular genetics to investigate the ARIH2 protein.

What are the consequences of ARIH2-mediated protein ubiquitination?

Ubiquitination by ARIH2 can lead to proteasomal degradation of the target protein, resulting in regulation of cellular processes, such as cell cycle progression, DNA repair, and immune responses.

Are there any inhibitors or activators discovered for ARIH2?

Currently, there are no specific inhibitors or activators known for ARIH2. However, research efforts are underway to identify small molecules or compounds that can modulate its activity for potential therapeutic purposes.

Are there any therapeutic implications of targeting ARIH2?

Targeting ARIH2 has shown potential for therapeutic intervention, particularly in the fields of cancer treatment and neurodegenerative disease research. However, further studies are needed to fully understand its potential as a therapeutic target.