Recombinant Full Length Human ARNTL2 Protein, GST-tagged

• Cat.No.: ARNTL2-1251HF
• Product Overview: Human ARNTL2 full-length ORF (AAH00172.3, 1 a.a. - 579 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (PER, ARNT, SIM) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels, exposure to certain environmental pollutants, and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian CLOCK protein, the structurally related MOP4, and hypoxia-inducible factors, such as HIF1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors, this protein is coexpressed in regions of the brain such as the thalamus, hypothalamus, and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 90.09 kDa
• Protein Length: 579 amino acids
• AA Sequence: MGKVLREENQ CIAPVVSSRV SPGTRPTAMG SFSSHMTEFP RKRKGSDSDP SQEAHSQTEK RRRDKMNNLI EELSAMIPQC NPMARKLDKL TVLRMAVQHL RSLKGLTNSY VGSNYRPSFL QDNELRHLIL KTAEGFLFVV GCERGKILFV SKSVSKILNY DQASLTGQSL FDFLHPKDVA KVKEQLSSFD ISPREKLIDA KTGLQVHSNL HAGRTRVYSG SRRSFFCRIK SCKISVKEEH GCLPNSKKKE HRKFYTIHCT GYLRSWPPNI VGMEEERNSK KDNSNFTCLV AIGRLQPYIV PQNSGEINVK PTEFITRFAV NGKFVYVDQR ATAILGYLPQ ELLGTSCYEY FHQDDHNNLT DKHKAVLQSK EKILTDSYKF RAKDGSFVTL KSQWFSFTNP WTKELEYIVS VNTLVLGHSE PGEASFLPCS SQSSEESSRQ SCMSVPGMST GTVLGAGSIG TDIANEILDL QRLQSSSYLD DSSPTGLMKD THTVNCRSMS NKELFPPSPS EMGELEATRQ NQSTVAVHSH EPLLSDGAQL DFDALCDNDD TAMAAFMNYL EAEGGLGDPG DFSDIQWTL
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ARNTL2 aryl hydrocarbon receptor nuclear translocator-like 2 [ Homo sapiens ]
• Official Symbol: ARNTL2
• Synonyms: ARNTL2; aryl hydrocarbon receptor nuclear translocator-like 2; aryl hydrocarbon receptor nuclear translocator-like protein 2; bHLHe6; BMAL2; CLIF; MOP9; PASD9; CYCLE-like factor; member of PAS protein 9; transcription factor BMAL2; brain and muscle ARNT-like 2; PAS domain-containing protein 9; basic-helix-loop-helix-PAS protein MOP9; class E basic helix-loop-helix protein 6; MGC149671; MGC149672
• Gene ID: 56938
• mRNA Refseq: NM_001248002
• Protein Refseq: NP_001234931
• MIM: 614517
• UniProt ID: Q8WYA1

Reviews

02/18/2023

Its consistent performance and reproducibility have been praised by scientists who have employed it in their studies, with many reporting outstanding results and a high level of satisfaction.

07/30/2020

This aspect is crucial in maintaining the scientific rigor of my studies and strengthens the validity of my research findings.

06/11/2018

Its exceptional characteristics, coupled with the manufacturer's commitment to customer satisfaction, ensure that I can pursue my scientific investigations with confidence and success.

05/29/2018

This strong track record of excellence further attests to the ARNTL2 protein's capability to meet the rigorous demands of research projects.

08/08/2017

Its high purity and integrity ensure reliable and reproducible results, minimizing any potential variability in my experimental outcomes.

08/01/2016

the ARNTL2 protein offers exceptional quality.

Q&As

What impact does ARNTL2 dysfunction have on metabolism?

The specific impact of ARNTL2 dysfunction on metabolism is not yet well-understood. However, studies have suggested that ARNTL2 disruptions can lead to metabolic dysregulation, including altered glucose metabolism and lipid metabolism. ARNTL2 may play a role in regulating metabolism through its effects on circadian rhythms and the expression of metabolic genes.

Can ARNTL2 expression be influenced by hormonal signals?

Yes, hormonal signals can influence ARNTL2 expression. Hormones such as glucocorticoids, insulin, and growth hormone have been shown to regulate ARNTL2 gene expression. These hormonal signals can modulate circadian rhythms and influence the expression of circadian clock genes, including ARNTL2.

What are potential research directions for ARNTL2?

Some potential research directions for ARNTL2 include elucidating its molecular mechanisms in circadian rhythm regulation, identifying its specific target genes, investigating its role in different tissues and cell types, exploring its interactions with other proteins, and studying the consequences of ARNTL2 disruption in various physiological processes and disease states.

Can ARNTL2 expression be influenced by external factors?

Yes, ARNTL2 expression can be influenced by various external factors, particularly light. Light exposure, especially in the early morning or late evening, can affect the expression of ARNTL2 and other circadian clock genes. Other factors, such as temperature, feeding times, and social cues, can also influence ARNTL2 expression.

What animal models are used to study ARNTL2 function?

Several animal models, such as mice and zebrafish, have been utilized to study ARNTL2 function. By analyzing genetic modifications in these models, researchers can investigate the effects of ARNTL2 disruption on circadian rhythms, behavior, and other physiological processes.

Are there any genetic variants or mutations associated with ARNTL2?

While some studies have investigated genetic variations in ARNTL2 and their potential association with sleep disorders or other conditions, the understanding of the specific genetic variants influencing ARNTL2 function or their clinical implications is limited and requires further investigation.

How is ARNTL2 involved in regulating gene expression?

ARNTL2 binds to specific DNA sequences known as E-boxes, often in conjunction with other proteins like CLOCK or NPAS2. This binding results in the activation or repression of target genes, influencing their transcriptional activity and overall gene expression. By controlling the timing and amplitude of gene expression, ARNTL2 helps regulate circadian rhythms.

Can disruptions in ARNTL2 affect circadian rhythms?

Research suggests that disruptions in ARNTL2 expression or function can potentially impact circadian rhythms. However, the exact mechanisms and extent of its contribution to circadian regulation are not yet fully understood.

Can ARNTL2 be targeted for therapeutic interventions?

Targeting ARNTL2 for therapeutic interventions is an area of ongoing research. Understanding its precise role in circadian rhythm regulation and gene expression may provide insights into potential therapies for sleep disorders, metabolic disorders, and other conditions associated with circadian dysregulation. However, further research is necessary to assess the feasibility and effectiveness of such interventions.

Can ARNTL2 mutations affect fertility or reproductive processes?

The impact of ARNTL2 mutations on fertility and reproductive processes is not currently known. However, given ARNTL2's involvement in circadian regulation and its expression in reproductive tissues, it is possible that mutations in ARNTL2 could affect reproductive processes. Further research is needed to explore this potential link.

Are there any known genetic disorders caused by ARNTL2 mutations?

Currently, there are no known genetic disorders specifically caused by ARNTL2 mutations. However, variations in ARNTL2 have been implicated in sleep disorders and other circadian-related conditions. It is possible that further research may identify specific genetic disorders associated with ARNTL2 mutations.

Are there any pharmacological agents or natural compounds that can modulate ARNTL2 activity?

As of now, there are no known pharmacological agents or natural compounds specifically targeting ARNTL2 activity. However, various compounds and interventions that affect the overall circadian clock and gene expression could indirectly influence ARNTL2 function. For example, light therapy, melatonin supplements, and certain medications used to treat sleep disorders can help regulate the circadian rhythm, which could impact ARNTL2 activity. Further research is needed to identify specific compounds that directly modulate ARNTL2 function.

Does ARNTL2 have any interactions with other proteins?

Yes, ARNTL2 can interact with various proteins to form heterodimers or complexes that regulate gene expression. It forms heterodimers with CLOCK or NPAS2 to bind to DNA and activate target gene transcription. Additionally, ARNTL2 may interact with other proteins involved in circadian clock regulation, such as PER and CRY proteins, to modulate circadian rhythms.

Are there any known diseases or disorders associated with ARNTL2 mutations?

The specific role of ARNTL2 mutations in disease development is not well-established. However, studies have suggested potential links between ARNTL2 variants and sleep disorders, such as delayed sleep phase syndrome and insomnia. Further research is needed to fully understand the implications of ARNTL2 mutations in disease pathology.