Recombinant Full Length Human ARPC5 Protein, GST-tagged

• Cat.No.: ARPC5-1099HF
• Product Overview: Human ARPC5 full-length ORF ( AAH57237, 1 a.a. - 154 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 42.68 kDa
• Protein Length: 154 amino acids
• AA Sequence: MSKNTVSSAR FRKVDVDEYD ENKFVDEEDG GDGQAGPDEG EVDSCLRHSI TGNMTAALQA ALKNPPINTK SQAVKDRAGS IVLKVLISFK ANDIEKAVQS LDKNGVDLLM KYIYKGFESP SDNSSAMLLQ WHEKALAAGG VGSIVRVLTA RKTV
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ARPC5 actin related protein 2/3 complex, subunit 5, 16kDa [ Homo sapiens ]
• Official Symbol: ARPC5
• Synonyms: ARPC5; actin related protein 2/3 complex, subunit 5, 16kDa; actin related protein 2/3 complex, subunit 5 (16 kD); actin-related protein 2/3 complex subunit 5; ARC16; Arp2/3 protein complex subunit p16; dJ127C7.3; p16 Arc; arp2/3 complex 16 kDa subunit; p16-Arc; MGC88523
• Gene ID: 10092
• mRNA Refseq: NM_005717
• Protein Refseq: NP_005708
• MIM: 604227
• UniProt ID: O15511

Reviews

06/26/2022

Its stability and compatibility make it an excellent tool for visualizing and characterizing protein structures at high resolution.

08/19/2020

the ARPC5 protein has proven to be invaluable in protein electron microscopy structure analysis.

03/20/2020

The ARPC5 protein stands out for its exceptional quality, making it an excellent choice to meet my experimental requirements.

Q&As

Is ARPC5 essential for embryonic development?

Studies in animal models have demonstrated that ARPC5 is essential for embryonic development. Knockout or depletion of ARPC5 leads to severe developmental defects, including abnormalities in neurodevelopment, highlighting its importance during embryogenesis.

Can alterations in ARPC5 expression or function affect cell migration?

Yes, alterations in ARPC5 expression or function can significantly impact cell migration. Since ARPC5 is a crucial component of the ARP2/3 complex involved in actin nucleation and branching, dysregulation of ARPC5 can lead to impaired cell migration and changes in cell morphology.

Are there any known post-translational modifications of ARPC5?

Currently, there is limited information available on the post-translational modifications of ARPC5. Further research is needed to investigate if ARPC5 undergoes any modifications like phosphorylation or ubiquitination, which could potentially regulate its activity and interactions.

Are there any known diseases associated with ARPC5 mutations?

To date, no specific diseases have been directly linked to ARPC5 mutations. However, given its involvement in actin dynamics and cellular processes, dysregulation of ARPC5 could potentially contribute to various diseases, including those involving developmental defects or abnormalities in cell migration.

What are some current areas of active research regarding ARPC5?

Current research on ARPC5 is focused on understanding its molecular mechanisms within the ARP2/3 complex, investigating its roles in cellular processes like cell migration and adhesion, and exploring its potential implications in disease progression. Additionally, there is ongoing research to identify specific regulators or modulators of ARPC5 activity for therapeutic purposes.

How does ARPC5 interact with the ARP2/3 complex?

ARPC5 interacts with other subunits of the ARP2/3 complex, including ARPC2 and ARPC4, forming a stable protein complex. This interaction is necessary for the proper functioning of the ARP2/3 complex in actin nucleation.

Can ARPC5 play a role in cellular adhesion?

Yes, ARPC5 can play a role in cellular adhesion through its involvement in actin dynamics. The ARP2/3 complex, including ARPC5, contributes to the formation of structures like lamellipodia and focal adhesions, which are essential for cell adhesion to the extracellular matrix.

Are there any known binding partners of ARPC5 besides the ARP2/3 complex?

Apart from its interaction with the ARP2/3 complex, ARPC5 can also interact with other actin-binding proteins, such as cortactin and N-WASP (Neuronal Wiskott-Aldrich Syndrome Protein). These interactions contribute to the regulation of actin dynamics and various cellular processes.

Does ARPC5 have any role in cancer progression or metastasis?

Yes, emerging evidence suggests that ARPC5 may play a role in cancer progression and metastasis. The dysregulation of actin dynamics, in which ARPC5 is involved, can affect cellular processes such as invasion and migration, which are crucial for cancer metastasis.