Recombinant Full Length Human ART4 Protein, GST-tagged

• Cat.No.: ART4-1019HF
• Product Overview: Human ART4 full-length ORF ( NP_066549.2, 1 a.a. - 314 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known. [provided by RefSeq, Jul 2008]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 62.3 kDa
• Protein Length: 314 amino acids
• AA Sequence: MGPLINRCKK ILLPTTVPPA TMRIWLLGGL LPFLLLLSGL QRPTEGSEVA IKIDFDFAPG SFDDQYQGCS KQVMEKLTQG DYFTKDIEAQ KNYFRMWQKA HLAWLNQGKV LPQNMTTTHA VAILFYTLNS NVHSDFTRAM ASVARTPQQY ERSFHFKYLH YYLTSAIQLL RKDSIMENGT LCYEVHYRTK DVHFNAYTGA TIRFGQFLST SLLKEEAQEF GNQTLFTIFT CLGAPVQYFS LKKEVLIPPY ELFKVINMSY HPRGDWLQLR STGNLSTYNC QLLKASSKKC IPDPIAIASL SFLTSVIIFS KSRV
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ART4 ADP-ribosyltransferase 4 (Dombrock blood group) [ Homo sapiens ]
• Official Symbol: ART4
• Synonyms: ART4; ADP-ribosyltransferase 4 (Dombrock blood group); ADP ribosyltransferase 4 , ADP ribosyltransferase 4 (DO blood group) , DO, Dombrock blood group; ecto-ADP-ribosyltransferase 4; CD297; DOK1; mono-ADP-ribosyltransferase 4; mono(ADP-ribosyl)transferase 4; Dombrock blood group carrier molecule; ADP-ribosyltransferase 4 (DO blood group); NAD(P)(+)--arginine ADP-ribosyltransferase 4; DO
• Gene ID: 420
• mRNA Refseq: NM_021071
• Protein Refseq: NP_066549
• MIM: 110600
• UniProt ID: Q93070

Reviews

01/04/2023

With its remarkable purity and reliability, coupled with the manufacturer's excellent technical support, this protein is a crucial asset in addressing my scientific inquiries and advancing my research goals.

06/17/2017

In addition to its outstanding quality, the Art4 Protein exhibits exceptional stability during storage and usage.

04/07/2016

Their team of experts has been instrumental in addressing any issues or concerns I have encountered during my experiments.

Q&As

Does ART4 have any non-enzymatic functions or interactions with other proteins?

Beyond its enzymatic role in ADP-ribosylation, ART4 does not have well-characterized non-enzymatic functions or specific protein interactions.

How does ART4 catalyze ADP-ribosylation?

ART4 transfers ADP-ribose from NAD+ to specific target proteins, modifying their structure and function.

Are there any known diseases or disorders associated with mutations in the ART4 gene?

Mutations in the ART4 gene are not commonly associated with diseases or disorders in humans.

Are there any therapeutic applications targeting ART4?

ART4 itself is not targeted therapeutically. However, understanding its role in blood group antigen regulation may have implications in blood transfusion medicine and compatibility testing.

What are some known substrates of ART4?

ART4 ADP-ribosylates specific glycoproteins on the surface of red blood cells, such as the P1 and P2 antigens.

Is the activity of ART4 influenced by any cofactors or regulators?

ART4 activity depends on the availability of NAD+ as a substrate, and it may also be regulated by cellular signaling pathways related to red blood cell function.