Recombinant Full Length Human ATP6V0C Protein, C-Flag-tagged

• Cat.No.: ATP6V0C-2129HFL
• Product Overview: Recombinant Full Length Human ATP6V0C Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 15.6 kDa
• AA Sequence: MSESKSGPEYASFFAVMGASAAMVF SALGAAYGTAKSGTGIAAMSVMRPE QIMKSIIPVVMAGIIAIYGL VVAVLIANSLNDDISLYKSFLQLGA GLSVGLSGLAAGFAIGIVGDAGVRG TAQQPRLFVGMILILIFAEV LGLYGLIVALILSTK TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

Gene Information

• Gene Name: ATP6V0C ATPase H+ transporting V0 subunit c [ Homo sapiens (human) ]
• Official Symbol: ATP6V0C
• Synonyms: ATPL; VATL; VPPC; Vma3; ATP6C; ATP6L
• Gene ID: 527
• mRNA Refseq: NM_001694.4
• Protein Refseq: NP_001685.1
• MIM: 108745
• UniProt ID: P27449