Recombinant Full Length Human ATP6V1B2 Protein, C-Flag-tagged
Cat.No. : | ATP6V1B2-1223HFL |
Product Overview : | Recombinant Full Length Human ATP6V1B2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells. |
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Description : | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. |
Source : | Mammalian cells |
Species : | Human |
Tag : | Flag |
Form : | 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Molecular Mass : | 56.3 kDa |
AA Sequence : | MALRAMRGIVNGAAPELPVPTGGPA VGAQEQALAVSRNYLSQPRLTYKTV SGVNGPLVILDHVKFPRYAE IVHLTLPDGTKRSGQVLEVSGSKAV VQVFEGTSGIDAKKTSCEFTGDILR TPVSEDMLGRVFNGSGKPID RGPVVLAEDFLDIMGQPINPQCRIY PEEMIRTGISAIDGMNSIARGQKIP IFSAAGLPHNEIAAQICRQA GLVKKSKDVVDYSEENFAIVFAAMG VNMETARFFKSDFEENGSMDNVCLF LNLANDPTIERIITPRLALT TAEFLAYQCEKHVLVILTDMSSYAE ALREVSAAREEVPGRRGFPGYMYTD LATIYERAGRVGGRNGSITQ IPILTMPNDDITHPIPDLTGYITEG QIYVDRQLHNRQIYPPINVLPSLSR LMKSAIGEGMTRKDHADVSN QLYACYAIGKDVQAMKAVVGEEALT SDDLLYLEFLQKFERNFIAQGPYEN RTVFETLDIGWQLLRIFPKE MLKRIPQSTLSEFYPRDSAKHTRTR PLEQKLISEEDLAANDILDYKDDDD KV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining. |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade. |
Concentration : | >50 ug/mL as determined by microplate BCA method. |
Preparation : | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Protein Families : | Druggable Genome |
Protein Pathways : | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
Gene Name : | ATP6V1B2 ATPase H+ transporting V1 subunit B2 [ Homo sapiens (human) ] |
Official Symbol : | ATP6V1B2 |
Synonyms : | DOOD; HO57; VATB; VPP3; Vma2; ZLS2; ATP6B2; ATP6B1B2 |
Gene ID : | 526 |
mRNA Refseq : | NM_001693.4 |
Protein Refseq : | NP_001684.2 |
MIM : | 606939 |
UniProt ID : | P21281 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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