Recombinant Full Length Human BDNF Protein, C-Flag-tagged

• Cat.No.: BDNF-454HFL
• Product Overview: Recombinant Full Length Human BDNF Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 25.7 kDa
• AA Sequence: MQSREEEWFHQVRRVMTILFLTMVI SYFGCMKAAPMKEANIRGQGGLAYP GVRTHGTLESVNGPKAGSRG LTSLADTFEHVIEELLDEDQKVRPN EENNKDADLYTSRVMLSSQVPLEPP LLFLLEEYKNYLDAANMSMR VRRHSDPARRGELSVCDSISEWVTA ADKKTAVDMSGGTVTVLEKVPVSKG QLKQYFYETKCNPMGYTKEG
  CRGIDKRHWNSQCRTTQSYVRALTM DSKKRIGWRFIRIDTSCVCTLTIKR GRTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Transmembrane
• Protein Pathways: Huntington's disease, MAPK signaling pathway, Neurotrophin signaling pathway

Gene Information

• Gene Name: BDNF brain derived neurotrophic factor [ Homo sapiens (human) ]
• Official Symbol: BDNF
• Synonyms: ANON2; BULN2
• Gene ID: 627
• mRNA Refseq: NM_170734.4
• Protein Refseq: NP_733930.1
• MIM: 113505
• UniProt ID: P23560