| Description : |
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Molecular Mass : |
84.3 kDa |
| AA Sequence : |
MAALSGGGGGGAEPGQALFNGDMEP EAGAGAGAAASSAADPAIPEEVWNI KQMIKLTQEHIEALLDKFGG EHNPPSIYLEAYEEYTSKLDALQQR EQQLLESLGNGTDFSVSSSASMDTV TSSSSSSLSVLPSSLSVFQN PTDVARSNPKSPQKPIVRVFLPNKQ RTVVPARCGVTVRDSLKKALMMRGL IPECCAVYRIQDGEKKPIGW DTDISWLTGEELHVEVLENVPLTTH NFVRKTFFTLAFCDFCRKLLFQGFR CQTCGYKFHQRCSTEVPLMC VNYDQLDLLFVSKFFEHHPIPQEEA SLAETALTSGSSPSAPASDSIGPQI LTSPSPSKSIPIPQPFRPAD EDHRNQFGQRDRSSSAPNVHINTIE PVNIDDLIRDQGFRGDGGSTTGLSA TPPASLPGSLTNVKALQKSP GPQRERKSSSSSEDRNRMKTLGRRD SSDDWEIPDGQITVGQRIGSGSFGT VYKGKWHGDVAVKMLNVTAP TPQQLQAFKNEVGVLRKTRHVNILL FMGYSTKPQLAIVTQWCEGSSLYHH LHIIETKFEMIKLIDIARQT AQGMDYLHAKSIIHRDLKSNNIFLH EDLTVKIGDFGLATVKSRWSGSHQF EQLSGSILWMAPEVIRMQDK NPYSFQSDVYAFGIVLYELMTGQLP YSNINNRDQIIFMVGRGYLSPDLSK VRSNCPKAMKRLMAECLKKK RDERPLFPQILASIELLARSLPKIH RSASEPSLNRAGFQTEDFSLYACAS PKTPIQAGGYGAFPVHTRTRPLEQK LISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome, Protein Kinase |
| Protein Pathways : |
Acute myeloid leukemia, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Thyroid cancer, Vascular smooth muscle contraction |
