Recombinant Full Length Human CHIT1 Protein, C-Flag-tagged

• Cat.No.: CHIT1-1899HFL
• Product Overview: Recombinant Full Length Human CHIT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 51.5 kDa
• AA Sequence: MVRSVAWAGFMVLLMIPWGSAAKLV CYFTNWAQYRQGEARFLPKDLDPSL CTHLIYAFAGMTNHQLSTTE WNDETLYQEFNGLKKMNPKLKTLLA IGGWNFGTQKFTDMVATANNRQTFV NSAIRFLRKYSFDGLDLDWE YPGSQGSPAVDKERFTTLVQDLANA FQQEAQTSGKERLLLSAAVPAGQTY VDAGYEVDKIAQNLDFVNLM AYDFHGSWEKVTGHNSPLYKRQEES GAAASLNVDAAVQQWLQKGTPASKL ILGMPTYGRSFTLASSSDTR VGAPATGSGTPGPFTKEGGMLAYYE VCSWKGATKQRIQDQKVPYIFRDNQ WVGFDDVESFKTKVSYLKQK GLGGAMVWALDLDDFAGFSCNQGRY PLIQTLRQELSLPYLPSGTPELEVP KPGQPSEPEHGPSPGQDTFC QGKADGLYPNPRERSSFYSCAAGRL FQQSCPTGLVFSNSCKCCTWN TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Amino sugar and nucleotide sugar metabolism

Gene Information

• Gene Name: CHIT1 chitinase 1 [ Homo sapiens (human) ]
• Official Symbol: CHIT1
• Synonyms: CHI3; CHIT; CHITD
• Gene ID: 1118
• mRNA Refseq: NM_003465.3
• Protein Refseq: NP_003456.1
• MIM: 600031
• UniProt ID: Q13231