Recombinant Full Length Human COL7A1 Protein, C-Flag-tagged

• Cat.No.: COL7A1-1006HFL
• Product Overview: Recombinant Full Length Human COL7A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 295.22 kDa
• AA Sequence: MTLRLLVAALCAGILAEAPRVRAQH RERVTCTRLYAADIVFLLDGSSSIG RSNFREVRSFLEGLVLPFSG AASAQGVRFATVQYSDDPRTEFGLD ALGSGGDVIRAIRELSYKGGNTRTG AAILHVADHVFLPQLARPGV PKVCILITDGKSQDLVDTAAQRLKG QGVKLFAVGIKNADPEELKRVASQP TSDFFFFVNDFSILRTLLPL VSRRVCTTAGGVPVTRPPDDSTSAP RDLVLSEPSSQSLRVQWTAASGPVT GYKVQYTPLTGLGQPLPSER QEVNVPAGETSVRLRGLRPLTEYQV TVIALYANSIGEAVSGTARTTALEG PELTIQNTTAHSLLVAWRSV PGATGYRVTWRVLSGGPTQQQELGP GQGSVLLRDLEPGTDYEVTVSTLFG RSVGPATSLMARTDASVEQT LRPVILGPTSILLSWNLVPEARGYR LEWRRETGLEPPQKVVLPSDVTRYQ LDGLQPGTEYRLTLYTLLEG HEVATPATVVPTGPELPVSPVTDLQ ATELPGQRVRVSWSPVPGATQYRII VRSTQGVERTLVLPGSQTAF DLDDVQAGLSYTVRVSARVGPREGS ASVLTVRREPETPLAVPGLRVVVSD ATRVRVAWGPVPGASGFRIS WSTGSGPESSQTLPPDSTATDITGL QPGTTYQVAVSVLRGREEGPAAVIV ARTDPLGPVRTVHVTQASSS SVTITWTRVPGATGYRVSWHSAHGP EKSQLVSGEATVAELDGLEPDTEYT VHVRAHVAGVDGPPASVVVR TAPEPVGRVSRLQILNASSDVLRIT WVGVTGATAYRLAWGRSEGGPMRHQ ILPGNTDSAEIRGLEGGVSY SVRVTALVGDREGTPVSIVVTTPPE APPALGTLHVVQRGEHSLRLRWEPV PRAQGFLLHWQPEGGQEQSR VLGPELSSYHLDGLEPATQYRVRLS VLGPAGEGPSAEVTARTESPRVPSI ELRVVDTSIDSVTLAWTPVS RASSYILSWRPLRGPGQEVPGSPQT LPGISSSQRVTGLEPGVSYIFSLTP VLDGVRGPEASVTQTPVCPR GLADVVFLPHATQDNAHRAEATRRV LERLVLALGPLGPQAVQVGLLSYSH RPSPLFPLNGSHDLGIILQR IRDMPYMDPSGNNLGTAVVTAHRYM LAPDAPGRRQHVPGVMVLLVDEPLR GDIFSPIREAQASGLNVVML GMAGADPEQLRRLAPGMDSVQTFFA VDDGPSLDQAVSGLATALCQASFTT QPRPEPCPVYCPKGQKGEPG EMGLRGQVGPPGDPGLPGRTGAPGP QGPPGSATAKGERGFPGADGRPGSP GRAGNPGTPGAPGLKGSPGL PGPRGDPGERGPRGPKGEPGAPGQV IGGEGPGLPGRKGDPGPSGPPGPRG PLGDPGPRGPPGLPGTAMKG DKGDRGERGPPGPGEGGIAPGEPGL PGLPGSPGPQGPVGPPGKKGEKGDS EDGAPGLPGQPGSPGEQGPR GPPGAIGPKGDRGFPGPLGEAGEKG ERGPPGPAGSRGLPGVAGRPGAKGP EGPPGPTGRQGEKGEPGRPG DPAVVGPAVAGPKGEKGDVGPAGPR GATGVQGERGPPGLVLPGDPGPKGD PGDRGPIGLTGRAGPPGDSG PPGEKGDPGRPGPPGPVGPRGRDGE VGEKGDEGPPGDPGLPGKAGERGLR GAPGVRGPVGEKGDQGDPGE DGRNGSPGSSGPKGDRGEPGPPGPP GRLVDTGPGAREKGEPGDRGQEGPR GPKGDPGLPGAPGERGIEGF RGPPGPQGDPGVRGPAGEKGDRGPP GLDGRSGLDGKPGAAGPSGPNGAAG KAGDPGRDGLPGLRGEQGLP GPSGPPGLPGKPGEDGKPGLNGKNG EPGDPGEDGRKGEKGDSGASGREGR DGPKGERGAPGILGPQGPPG LPGPVGPPGQGFPGVPGGTGPKGDR GETGSKGEQGLPGERGLRGEPGSVP NVDRLLETAGIKASALREIV ETWDESSGSFLPVPERRRGPKGDSG EQGPPGKEGPIGFPGERGLKGDRGD PGPQGPPGLALGERGPPGPS GLAGEPGKPGIPGLPGRAGGVGEAG RPGERGERGEKGERGEQGRDGPPGL PGTPGPPGPPGPKVSVDEPG PGLSGEQGPPGLKGAKGEPGSNGDQ GPKGDRGVPGIKGDRGEPGPRGQDG NPGLPGERGMAGPEGKPGLQ GPRGPPGPVGGHGDPGPPGAPGLAG PAGPQGPSGLKGEPGETGPPGRGLT GPTGAVGLPGPPGPSGLVGP QGSPGLPGQVGETGKPGAPGRDGAS GKDGDRGSPGVPGSPGLPGPVGPKG EPGPTGAPGQAVVGLPGAKG EKGAPGGLAGDLVGEPGAKGDRGLP GPRGEKGEAGRAGEPGDPGEDGQKG APGPKGFKGDPGVGVPGSPG PPGPPGVKGDLGLPGLPGAPGVVGF PGQTGPRGEMGQPGPSGERGLAGPP GREGIPGPLGPPGPPGSVGP PGASGLKGDKGDPGVGLPGPRGERG EPGIRGEDGRPGQEGPRGLTGPPGS RGERGEKGDVGSAGLKGDKG DSAVILGPPGPRGAKGDMGERGPRG LDGDKGPRGDNGDPGDKGSKGEPGD KGSAGLPGLRGLLGPQGQPG AAGIPGDPGSPGKDGVPGIRGEKGD VGFMGPRGLKGERGVKGACGLDGEK GDKGEAGPPGRPGLAGHKGE MGEPGVPGQSGAPGKEGLIGPKGDR GFDGQPGPKGDQGEKGERGTPGIGG FPGPSGNDGSAGPPGPPGSV GPRGPEGLQGQKGERGPPGERVVGA PGVPGAPGERGEQGRPGPAGPRGEK GEAALTEDDIRGFVRQEMSQ HCACQGQFIASGSRPLPSYAADTAG SQLHAVPVLRVSHAEEEERVPPEDD EYSEYSEYSVEEYQDPEAPW DSDDPCSLPLDEGSCTAYTLRWYHR AVTGSTEACHPFVYGGCGGNANRFG TREACERRCPPRVVQSQGTG
  TAQDTRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: COL7A1 collagen type VII alpha 1 chain [ Homo sapiens (human) ]
• Official Symbol: COL7A1
• Synonyms: EBD1; EBR1; EBDCT; NDNC8
• Gene ID: 1294
• mRNA Refseq: NM_000094.4
• Protein Refseq: NP_000085.1
• MIM: 120120
• UniProt ID: Q02388

Reviews

06/24/2023

COL7A1 is less polluting, safe and hygienic, and suitable for long-term use.

02/13/2022

It is stable under different temperature and pH conditions.

05/09/2020

Their service attitude is very good and always patient to answer our questions.

Q&As

How is the COL7A1 gene mutation diagnosed?

It can be diagnosed through genetic testing and skin biopsies. Genetic testing can detect mutations in the COL7A1 gene, while skin biopsies can observe abnormalities in skin structure.

What are the treatments for COL7A1 gene mutations?

There is currently no cure for the COL7A1 gene mutation, but there are treatments that can relieve symptoms, such as the use of special dressings, medication, and surgery.

What types of skin damage is COL7A1 associated with?

The COL7A1 gene has been implicated in a variety of skin injuries, including burns, trauma, and surgical trauma. These injuries may lead to skin complications in DEB patients.

What happens to a mutation in the COL7A1 gene?

Mutations in the COL7A1 gene can cause structural abnormalities in the skin, making it fragile and prone to blisters and scarring, which can be accompanied by abnormalities in bones and internal organs.

Which drug responses are involved in the COL7A1 gene?

Certain drugs may have efficacy or side effects on the COL7A1 gene mutation, for example, certain immunosuppressants used to treat other skin conditions may have adverse effects on the skin of DEB patients.

How to improve the COL7A1 gene mutation through diet?

Although there is no conclusive evidence that diet can improve the COL7A1 gene mutation, maintaining a balanced diet with adequate protein, vitamins and minerals is beneficial for overall health.