Recombinant Full Length Human CYP46A1 Protein, C-Flag-tagged

• Cat.No.: CYP46A1-221HFL
• Product Overview: Recombinant Full Length Human CYP46A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 56.6 kDa
• AA Sequence: MSPGLLLLGSAVLLAFGLCCTFVHR ARSRYEHIPGPPRPSFLLGHLPCFW KKDEVGGRVLQDVFLDWAKK YGPVVRVNVFHKTSVIVTSPESVKK FLMSTKYNKDSKMYRALQTVFGERL FGQGLVSECNYERWHKQRRV IDLAFSRSSLVSLMETFNEKAEQLV EILEAKADGQTPVSMQDMLTYTAMD ILAKAAFGMETSMLLGAQKP LSQAVKLMLEGITASRNTLAKFLPG KRKQLREVRESIRFLRQVGRDWVQR RREALKRGEEVPADILTQIL KAEEGAQDDEGLLDNFVTFFIAGHE TSANHLAFTVMELSRQPEIVARLQA EVDEVIGSKRYLDFEDLGRL QYLSQVLKESLRLYPPAWGTFRLLE EETLIDGVRVPGNTPLLFSTYVMGR MDTYFEDPLTFNPDRFGPGA PKPRFTYFPFSLGHRSCIGQQFAQM EVKVVMAKLLQRLEFRLVPGQRFGL QEQATLKPLDPVLCTLRPRG
  WQPAPPPPPCTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, P450
• Protein Pathways: Primary bile acid biosynthesis

Gene Information

• Gene Name: CYP46A1 cytochrome P450 family 46 subfamily A member 1 [ Homo sapiens (human) ]
• Official Symbol: CYP46A1
• Synonyms: CP46; CYP46
• Gene ID: 10858
• mRNA Refseq: NM_006668.2
• Protein Refseq: NP_006659.1
• MIM: 604087
• UniProt ID: Q9Y6A2