Recombinant Full Length Human DBR1 Protein, C-Flag-tagged

• Cat.No.: DBR1-428HFL
• Product Overview: Recombinant Full Length Human DBR1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 61.4 kDa
• AA Sequence: MRVAVAGCCHGELDKIYETLALAER RGPGPVDLLLCCGDFQAVRNEADLR CMAVPPKYRHMQTFYRYYSG EKKAPVLTLFIGGNHEASNHLQELP YGGWVAPNIYYLGLAGVVKYRGVRI GGISGIFKSHDYRKGHFECP PYNSSTIRSIYHVRNIEVYKLKQLK QPIDIFLSHDWPRSIYHYGNKKQLL KTKSFFRQEVENNTLGSPAA SELLEHLKPTYWFSAHLHVKFAALM QHQAKDKGQTARATKFLALDKCLPH RDFLQILEIEHDPSAPDYLE YDIEWLTILRATDDLINVTGRLWNM PENNGLHARWDYSATEEGMKEVLEK LNHDLKVPCNFSVTAACYDP SKPQTQMQLIHRINPQTTEFCAQLG IIDINVRLQKSKEEHHVCGEYEEQD DVESNDSGEDQSEYNTDTSA LSSINPDEIMLDEEEDEDSIVSAHS GMNTPSVEPSDQASEFSASFSDVRI LPGSMIVSSDDTVDSTIDRE
  GKPGGTVESGNGEDLTKVPLKRLSD EHEPEQRKKIKRRNQAIYAAVDDDD DDAATRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: DBR1 debranching RNA lariats 1 [ Homo sapiens (human) ]
• Official Symbol: DBR1
• Synonyms: debranching enzyme homolog 1; debranching enzyme homolog 1 (S. cerevisiae); RNA lariat debranching enzyme
• Gene ID: 51163
• mRNA Refseq: NM_016216.4
• Protein Refseq: NP_057300.2
• MIM: 51163
• UniProt ID: Q9UK59