Recombinant Full Length Human DICER1 Protein, C-Flag-tagged

• Cat.No.: DICER1-1120HFL
• Product Overview: Recombinant Full Length Human DICER1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. This protein also acts as a strong antiviral agent with activity against RNA viruses, including the Zika and SARS-CoV-2 viruses. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 218.5 kDa
• AA Sequence: MKSPALQPLSMAGLQLMTPASSPMG PFFGLPWQQEAIHDNIYTPRKYQVE LLEAALDHNTIVCLNTGSGK TFIAVLLTKELSYQIRGDFSRNGKR TVFLVNSANQVAQQVSAVRTHSDLK VGEYSNLEVNASWTKERWNQ EFTKHQVLIMTCYVALNVLKNGYLS LSDINLLVFDECHLAILDHPYREIM KLCENCPSCPRILGLTASIL NGKCDPEELEEKIQKLEKILKSNAE TATDLVVLDRYTSQPCEIVVDCGPF TDRSGLYERLLMELEEALNF INDCNISVHSKERDSTLISKQILSD CRAVLVVLGPWCADKVAGMMVRELQ KYIKHEQEELHRKFLLFTDT FLRKIHALCEEHFSPASLDLKFVTP KVIKLLEILRKYKPYERQQFESVEW YNNRNQDNYVSWSDSEDDDE DEEIEEKEKPETNFPSPFTNILCGI IFVERRYTAVVLNRLIKEAGKQDPE LAYISSNFITGHGIGKNQPR NKQMEAEFRKQEEVLRKFRAHETNL LIATSIVEEGVDIPKCNLVVRFDLP TEYRSYVQSKGRARAPISNY IMLADTDKIKSFEEDLKTYKAIEKI LRNKCSKSVDTGETDIDPVMDDDDV FPPYVLRPDDGGPRVTINTA IGHINRYCARLPSDPFTHLAPKCRT RELPDGTFYSTLYLPINSPLRASIV GPPMSCVRLAERVVALICCE KLHKIGELDDHLMPVGKETVKYEEE LDLHDEEETSVPGRPGSTKRRQCYP KAIPECLRDSYPRPDQPCYL YVIGMVLTTPLPDELNFRRRKLYPP EDTTRCFGILTAKPIPQIPHFPVYT RSGEVTISIELKKSGFMLSL QMLELITRLHQYIFSHILRLEKPAL EFKPTDADSAYCVLPLNVVNDSSTL DIDFKFMEDIEKSEARIGIP STKYTKETPFVFKLEDYQDAVIIPR YRNFDQPHRFYVADVYTDLTPLSKF PSPEYETFAEYYKTKYNLDL TNLNQPLLDVDHTSSRLNLLTPRHL NQKGKALPLSSAEKRKAKWESLQNK QILVPELCAIHPIPASLWRK AVCLPSILYRLHCLLTAEELRAQTA SDAGVGVRSLPADFRYPNLDFGWKK SIDSKSFISISNSSSAENDN YCKHSTIVPENAAHQGANRTSSLEN HDQMSVNCRTLLSESPGKLHVEVSA DLTAINGLSYNQNLANGSYD LANRDFCQGNQLNYYKQEIPVQPTT SYSIQNLYSYENQPQPSDECTLLSN KYLDGNANKSTSDGSPVMAV MPGTTDTIQVLKGRMDSEQSPSIGY SSRTLGPNPGLILQALTLSNASDGF NLERLEMLGDSFLKHAITTY LFCTYPDAHEGRLSYMRSKKVSNCN LYRLGKKKGLPSRMVVSIFDPPVNW LPPGYVVNQDKSNTDKWEKD EMTKDCMLANGKLDEDYEEEDEEEE SLMWRAPKEEADYEDDFLEYDQEHI RFIDNMLMGSGAFVKKISLS PFSTTDSAYEWKMPKKSSLGSMPFS SDFEDFDYSSWDAMCYLDPSKAVEE DDFVVGFWNPSEENCGVDTG KQSISYDLHTEQCIADKSIADCVEA LLGCYLTSCGERAAQLFLCSLGLKV LPVIKRTDREKALCPTRENF NSQQKNLSVSCAAASVASSRSSVLK DSEYGCLKIPPRCMFDHPDADKTLN HLISGFENFEKKINYRFKNK AYLLQAFTHASYHYNTITDCYQRLE FLGDAILDYLITKHLYEDPRQHSPG VLTDLRSALVNNTIFASLAV KYDYHKYFKAVSPELFHVIDDFVQF QLEKNEMQGMDSELRRSEEDEEKEE DIEVPKAMGDIFESLAGAIY MDSGMSLETVWQVYYPMMRPLIEKF SANVPRSPVRELLEMEPETAKFSPA ERTYDGKVRVTVEVVGKGKF
  KGVGRSYRIAKSAAARRALRSLKAN QPQVPNSTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: DICER1 dicer 1, ribonuclease III [ Homo sapiens (human) ]
• Official Symbol: DICER1
• Synonyms: DCR1; GLOW; MNG1; aviD; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
• Gene ID: 23405
• mRNA Refseq: NM_030621.4
• Protein Refseq: NP_085124.2
• MIM: 606241
• UniProt ID: Q9UPY3

Reviews

04/26/2023

Its purity and functional integrity guarantee optimal performance in a wide range of experimental applications.

10/26/2018

Their team of experts is readily available to assist you with any inquiries or challenges you may encounter during your experiments.

03/28/2018

This protein, manufactured with utmost precision, provides reliable and consistent results, ensuring the accuracy and reproducibility of your research findings.

Q&As

Can DICER1 mutations affect non-cancer-related health conditions?

DICER1 mutations may also lead to non-cancer conditions, such as pleuropulmonary blastoma syndrome and cystic nephroma.

Is there any potential for using DICER1 in targeted cancer therapies?

Research is ongoing to explore whether targeting DICER1-related pathways could lead to novel cancer treatments.

Are there any clinical tests or assays that measure DICER1 levels in patients?

Yes, some diagnostic tests can measure DICER1 expression, which may be relevant in certain cancer types.

Can DICER1 mutations be hereditary?

Yes, DICER1 mutations can be passed down through families and cause a hereditary predisposition to certain cancers.

How is DICER1 involved in cancer development and progression?

DICER1 mutations or dysregulation can lead to abnormal microRNA processing, contributing to the development of cancer.