Recombinant Full Length Human DNAJB1 Protein, C-Flag-tagged

• Cat.No.: DNAJB1-1382HFL
• Product Overview: Recombinant Full Length Human DNAJB1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.9 kDa
• AA Sequence: MGKDYYQTLGLARGASDEEIKRAYR RQALRYHPDKNKEPGAEEKFKEIAE AYDVLSDPRKREIFDRYGEE GLKGSGPSGGSGGGANGTSFSYTFH GDPHAMFAEFFGGRNPFDTFFGQRN GEEGMDIDDPFSGFPMGMGG FTNVNFGRSRSAQEPARKKQDPPVT HDLRVSLEEIYSGCTKKMKISHKRL NPDGKSIRNEDKILTIEVKK GWKEGTKITFPKEGDQTSNNIPADI VFVLKDKPHNIFKRDGSDVIYPARI SLREALCGCTVNVPTLDGRT
  IPVVFKDVIRPGMRRKVPGEGLPLP KTPEKRGDLIIEFEVIFPERIPQTS RTVLEQVLPI
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: DNAJB1 DnaJ heat shock protein family (Hsp40) member B1 [ Homo sapiens (human) ]
• Official Symbol: DNAJB1
• Synonyms: Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• Gene ID: 3337
• mRNA Refseq: NM_006145.3
• Protein Refseq: NP_006136.1
• MIM: 604572
• UniProt ID: P25685

Reviews

07/15/2022

Efficient peptide synthesis, crucial for advancing our peptide research.

07/21/2021

Exceptional protein engineering services, a vital resource for our lab.

11/26/2020

Reliable protein crystallography, a crucial part of structural research.

Q&As

How does DNAJB1 contribute to the pathogenesis of neurodegenerative diseases, such as Alzheimer's and Parkinson's?

DNAJB1 plays a role in neurodegenerative diseases by influencing the handling of misfolded proteins in neurons.

How does DNAJB1 influence the immune response, especially in regulating inflammation and immune cell function?

DNAJB1 modulates the immune system, particularly in controlling inflammation and immune cell responses.

How does DNAJB1 assist in protein folding and prevent misfolding or aggregation of proteins within cells?

DNAJB1 aids in protein folding and prevents the aggregation of misfolded proteins, crucial for maintaining cellular proteostasis.

What are the mechanisms by which DNAJB1 modulates apoptosis and cell survival under stress?

DNAJB1 regulates apoptosis and cell survival, especially under conditions of cellular stress.

What is the impact of DNAJB1 on cancer cell survival, proliferation, and response to chemotherapy?

DNAJB1 affects cancer cell behavior, influencing tumor growth, survival, and sensitivity to treatments.

What role does DNAJB1 play in cellular responses to heat shock and other stress conditions?

DNAJB1 is key in cellular stress responses, particularly to heat shock, by stabilizing proteins and aiding in recovery.

How do alterations in DNAJB1 expression or function affect muscle cell health and the development of muscular disorders?

Changes in DNAJB1 expression impact muscle cell function and health, potentially contributing to muscular disorders.