Recombinant Full Length Human DUSP12 Protein, C-Flag-tagged

• Cat.No.: DUSP12-1664HFL
• Product Overview: Recombinant Full Length Human DUSP12 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product is the human ortholog of the Saccharomyces cerevisiae YVH1 protein tyrosine phosphatase. It is localized predominantly in the nucleus, and is novel in that it contains, and is regulated by a zinc finger domain.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.5 kDa
• AA Sequence: MLEAPGPSDGCELSNPSASRVSCAG QMLEVQPGLYFGGAAAVAEPDHLRE AGITAVLTVDSEEPSFKAGP GVEDLWRLFVPALDKPETDLLSHLD RCVAFIGQARAEGRAVLVHCHAGVS RSVAIITAFLMKTDQLPFEK AYEKLQILKPEAKMNEGFEWQLKLY QAMGYEVDTSSAIYKQYRLQKVTEK YPELQNLPQELFAVDPTTVS QGLKDEVLYKCRKCRRSLFRSSSIL DHREGSGPIAFAHKRMTPSSMLTTG RQAQCTSYFIEPVQWMESAL
  LGVMDGQLLCPKCSAKLGSFNWYGE QCSCGRWITPAFQIHKNRVDEMKIL PVLGSQTGKITRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Phosphatase

Gene Information

• Gene Name: DUSP12 dual specificity phosphatase 12 [ Homo sapiens (human) ]
• Official Symbol: DUSP12
• Synonyms: YVH1; DUSP1
• Gene ID: 11266
• mRNA Refseq: NM_007240.3
• Protein Refseq: NP_009171.1
• MIM: 604835
• UniProt ID: Q9UNI6