Recombinant Full Length Human ECHS1 Protein, C-Flag-tagged

• Cat.No.: ECHS1-1352HFL
• Product Overview: Recombinant Full Length Human ECHS1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 31.2 kDa
• AA Sequence: MAALRVLLSCVRGPLRPPVRCPAWR PFASGANFEYIIAEKRGKNNTVGLI QLNRPKALNALCDGLIDELN QALKIFEEDPAVGAIVLTGGDKAFA AGADIKEMQNLSFQDCYSSKFLKHW DHLTQVKKPVIAAVNGYAFG GGCELAMMCDIIYAGEKAQFAQPEI LIGTIPGAGGTQRLTRAVGKSLAME MVLTGDRISAQDAKQAGLVS KICPVETLVEEAIQCAEKIASNSKI VVAMAKESVNAAFEMTLTEGSKLEK KLFYSTFATDDRKEGMTAFV
  EKRKANFKDQTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: beta-Alanine metabolism, Butanoate metabolism, Fatty acid elongation in mitochondria, Fatty acid metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation

Gene Information

• Gene Name: ECHS1 enoyl-CoA hydratase, short chain 1 [ Homo sapiens (human) ]
• Official Symbol: ECHS1
• Synonyms: SCEH; mECH; mECH1; ECHS1D
• Gene ID: 1892
• mRNA Refseq: NM_004092.4
• Protein Refseq: NP_004083.3
• MIM: 602292
• UniProt ID: P30084