Recombinant Full Length Human EHD2 Protein, C-Flag-tagged

• Cat.No.: EHD2-2173HFL
• Product Overview: Recombinant Full Length Human EHD2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 61 kDa
• AA Sequence: MFSWLKRGGARGQQPEAIRTVTSAL KELYRTKLLPLEEHYRFGAFHSPAL EDADFDGKPMVLVAGQYSTG KTSFIQYLLEQEVPGSRVGPEPTTD CFVAVMHGDTEGTVPGNALVVDPDK PFRKLNPFGNTFLNRFMCAQ LPNQVLESISIIDTPGILSGAKQRV SRGYDFPAVLRWFAERVDLIILLFD AHKLEISDEFSEAIGALRGH EDKIRVVLNKADMVETQQLMRVYGA LMWALGKVVGTPEVLRVYIGSFWSQ PLLVPDNRRLFELEEQDLFR DIQGLPRHAALRKLNDLVKRARLVR VHAYIISYLKKEMPSVFGKENKKKQ LILKLPVIFAKIQLEHHISP GDFPDCQKMQELLMAHDFTKFHSLK PKLLEALDEMLTHDIAKLMPLLRQE ELESTEVGVQGGAFEGTHMG PFVERGPDEAMEDGEEGSDDEAEWV VTKDKSKYDEIFYNLAPADGKLSGS KAKTWMVGTKLPNSVLGRIW KLSDVDRDGMLDDEEFALASHLIEA KLEGHGLPANLPRRLVPPSKRRHKG SAE TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Endocytosis

Gene Information

• Gene Name: EHD2 EH domain containing 2 [ Homo sapiens (human) ]
• Official Symbol: EHD2
• Synonyms: PAST2
• Gene ID: 30846
• mRNA Refseq: NM_014601.4
• Protein Refseq: NP_055416.2
• MIM: 605890
• UniProt ID: Q9NZN4