Recombinant Full Length Human EIF4G1 Protein, C-Flag-tagged

• Cat.No.: EIF4G1-1009HFL
• Product Overview: Recombinant Full Length Human EIF4G1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 175.3 kDa
• AA Sequence: MNKAPQSTGPPPAPSPGLPQPAFPP GQTAPVVFSTPQATQMNTPSQPRQH FYPSRAQPPSSAASRVQSAA PARPGPAAHVYPAGSQVMMIPSQIS YPASQGAYYIPGQGRSTYVVPTQQY PVQPGAPGFYPGASPTEFGT YAGAYYPAQGVQQFPTGVAPAPVLM NQPPQIAPKRERKTIRIRDPNQGGK DITEEIMSGARTASTPTPPQ TGGGLEPQANGETPQVAVIVRPDDR SQGAIIADRPGLPGPEHSPSESQPS SPSPTPSPSPVLEPGSEPNL AVLSIPGDTMTTIQMSVEESTPISR ETGEPYRLSPEPTPLAEPILEVEVT LSKPVPESEFSSSPLQAPTP LASHTVEIHEPNGMVPSEDLEPEVE SSPELAPPPACPSESPVPIAPTAQP EELLNGAPSPPAVDLSPVSE PEEQAKEVTASMAPPTIPSATPATA PSATSPAQEEEMEEEEEEEEGEAGE AGEAESEKGGEELLPPESTP IPANLSQNLEAAAATQVAVSVPKRR RKIKELNKKEAVGDLLDAFKEANPA VPEVENQPPAGSNPGPESEG SGVPPRPEEADETWDSKEDKIHNAE NIQPGEQKYEYKSDQWKPLNLEEKK RYDREFLLGFQFIFASMQKP EGLPHISDVVLDKANKTPLRPLDPT RLQGINCGPDFTPSFANLGRTTLST RGPPRGGPGGELPRGPAGLG PRRSQQGPRKEPRKIIATVLMTEDI KLNKAEKAWKPSSKRTAADKDRGEE DADGSKTQDLFRRVRSILNK LTPQMFQQLMKQVTQLAIDTEERLK GVIDLIFEKAISEPNFSVAYANMCR CLMALKVPTTEKPTVTVNFR KLLLNRCQKEFEKDKDDDEVFEKKQ KEMDEAATAEERGRLKEELEEARDI ARRRSLGNIKFIGELFKLKM LTEAIMHDCVVKLLKNHDEESLECL CRLLTTIGKDLDFEKAKPRMDQYFN QMEKIIKEKKTSSRIRFMLQ DVLDLRGSNWVPRRGDQGPKTIDQI HKEAEMEEHREHIKVQQLMAKGSDK RRGGPPGPPISRGLPLVDDG GWNTVPISKGSRPIDTSRLTKITKP GSIDSNNQLFAPGGRLSWGKGSSGG SGAKPSDAASEAARPATSTL NRFSALQQAVPTESTDNRRVVQRSS LSRERGEKAGDRGDRLERSERGGDR GDRLDRARTPATKRSFSKEV EERSRERPSQPEGLRKAASLTEDRD RGRDAVKREAALPPVSPLKAALSEE ELEKKSKAIIEEYLHLNDMK EAVQCVQELASPSLLFIFVRHGVES TLERSAIAREHMGQLLHQLLCAGHL STAQYYQGLYEILELAEDME IDIPHVWLYLAELVTPILQEGGVPM GELFREITKPLRPLGKAASLLLEIL GLLCKSMGPKKVGTLWREAG LSWKEFLPEGQDIGAFVAEQKVEYT LGEESEAPGQRALPSEELNRQLEKL LKEGSSNQRVFDWIEANLSE QQIVSNTLVRALMTAVCYSAIIFET PLRVDVAVLKARAKLLQKYLCDEQK ELQALYALQALVVTLEQPPN
  LLRMFFDALYDEDVVKEDAFYSWES SKDPAEQQGKGVALKSVTAFFKWLR EAEEESDHNTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Viral myocarditis

Gene Information

• Gene Name: EIF4G1 eukaryotic translation initiation factor 4 gamma 1 [ Homo sapiens (human) ]
• Official Symbol: EIF4G1
• Synonyms: P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1
• Gene ID: 1981
• mRNA Refseq: NM_198241.3
• Protein Refseq: NP_937884.2
• MIM: 600495
• UniProt ID: Q04637