Recombinant Full Length Human ENTPD4 Protein, C-Flag-tagged

• Cat.No.: ENTPD4-1858HFL
• Product Overview: Recombinant Full Length Human ENTPD4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 70.1 kDa
• AA Sequence: MGRIGISCLFPASWHFSISPVGCPR ILNTNLRQIMVISVLAAAVSLLYFS VVIIRNKYGRLTRDKKFQRY LARVTDIEATDTNNPNVNYGIVVDC GSSGSRVFVYCWPRHNGNPHDLLDI RQMRDKNRKPVVMKIKPGIS EFATSPEKVSDYISPLLNFAAEHVP RAKHKETPLYILCTAGMRILPESQQ KAILEDLLTDIPVHFDFLFS DSHAEVISGKQEGVYAWIGINFVLG RFEHIEDDDEAVVEVNIPGSESSEA IVRKRTAGILDMGGVSTQIA YEVPKTVSFASSQQEEVAKNLLAEF NLGCDVHQTEHVYRVYVATFLGFGG NAARQRYEDRIFANTIQKNR LLGKQTGLTPDMPYLDPCLPLDIKD EIQQNGQTIYLRGTGDFDLCRETIQ PFMNKTNETQTSLNGVYQPP IHFQNSEFYGFSEFYYCTEDVLRMG GDYNAAKFTKAAKDYCATKWSILRE RFDRGLYASHADLHRLKYQC FKSAWMFEVFHRGFSFPVNYKSLKT ALQVYDKEVQWTLGAILYRTRFLPL RDIQQEAFRASHTHWRGVSF VYNHYLFSGCFLVVLLAILLYLLRL RRIHRRTPRSSSAAALWMEEGLPAQ NAPGTL TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane
• Protein Pathways: Lysosome, Purine metabolism, Pyrimidine metabolism

Gene Information

• Gene Name: ENTPD4 ectonucleoside triphosphate diphosphohydrolase 4 [ Homo sapiens (human) ]
• Official Symbol: ENTPD4
• Synonyms: LAP70; LALP70; LYSAL1; UDPase; NTPDase-4
• Gene ID: 9583
• mRNA Refseq: NM_004901.5
• Protein Refseq: NP_004892.1
• MIM: 607577
• UniProt ID: Q9Y227