Recombinant Full Length Human EPB49 Protein, GST-tagged

• Cat.No.: EPB49-4274HF
• Product Overview: Human EPB49 full-length ORF ( AAH52805.1, 1 a.a. - 383 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene is an actin binding and bundling protein that plays a structural role in erythrocytes, by stabilizing and attaching the spectrin/actin cytoskeleton to the erythrocyte membrane in a phosphorylation-dependent manner. This protein contains a core domain in the N-terminus, and a headpiece domain in the C-terminus that binds F-actin. When purified from erythrocytes, this protein exists as a trimer composed of two 48 kDa polypeptides and a 52 kDa polypeptide. The different subunits arise from alternative splicing in the 3' coding region, where the headpiece domain is located. Disruption of this gene has been correlated with the autosomal dominant Marie Unna hereditary hypotrichosis disease, while loss of heterozygosity of this gene is thought to play a role in prostate cancer progression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 69.5 kDa
• Protein Length: 383 amino acids
• AA Sequence: MERLQKQPLTSPGSVSPSRDSSVPG SPSSIVAKMDNQVLGYKDLAAIPKD KAILDIERPDLMIYEPHFTYSLLEH VELPRSRERSLSPKSTSPPPSPEVW ADSRSPGIISQASAPRTTGTPRTSL PHFHHPETSRPDSNIYKKPPIYKQR ESVGGSPQTKHLIEDLIIESSKFPA AQPPDPNQPAKIETDYWPCPPSLAV VETEWRKRKASRRGAEEEEEEEDDD SGEEMKALRERQREELSKVTSNLGK MILKEEMEKSLPIRRKTRSLPDRTP FHTSLHQGTSKSSSLPAYGRTTLSR LQSTEFSPSGSETGSPGLQIYPYEM LVVTNKGRTKLPPGVDRMRLERHLS AEDFSRVFAMSPEEFGKLALWKRNE LKKKASLF
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: EPB49 erythrocyte membrane protein band 4.9 (dematin) [ Homo sapiens ]
• Official Symbol: EPB49
• Synonyms: EPB49; erythrocyte membrane protein band 4.9 (dematin); dematin; DMT; FLJ78462; FLJ98848
• Gene ID: 2039
• mRNA Refseq: NM_001114135
• Protein Refseq: NP_001107607
• MIM: 125305
• UniProt ID: Q08495