Recombinant Full Length Human FECH Protein, C-Flag-tagged

• Cat.No.: FECH-1762HFL
• Product Overview: Recombinant Full Length Human FECH Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 42.1 kDa
• AA Sequence: MRSLGANMAAALRAAGVLLRDPLAS SSWRVCQPWRWKSGAAAAAVTTETA QHAQGAKPQVQPQKRKPKTG ILMLNMGGPETLGDVHDFLLRLFLD RDLMTLPIQNKLAPFIAKRRTPKIQ EQYRRIGGGSPIKIWTSKQG EGMVKLLDELSPNTAPHKYYIGFRY VHPLTEEAIEEMERDGLERAIAFTQ YPQYSCSTTGSSLNAIYRYY NQVGRKPTMKWSTIDRWPTHHLLIQ CFADHILKELDHFPLEKRSEVVILF SAHSLPMSVVNRGDPYPQEV SATVQKVMERLEYCNPYRLVWQSKV GPMPWLGPQTDESIKGLCERGRKNI LLVPIAFTSDHIETLYELDI EYSQVLAKECGVENIRRAESLNGNP LFSKALADLVHSHIQSNELCSKQLT LSCPLCVNPVCRETKSFFTS QQL TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Porphyrin and chlorophyll metabolism

Gene Information

• Gene Name: FECH ferrochelatase [ Homo sapiens (human) ]
• Official Symbol: FECH
• Synonyms: EPP; FCE; EPP1
• Gene ID: 2235
• mRNA Refseq: NM_000140.5
• Protein Refseq: NP_000131.2
• MIM: 612386
• UniProt ID: P22830