Recombinant Full Length Human GAD2 Protein, C-Flag-tagged

• Cat.No.: GAD2-517HFL
• Product Overview: Recombinant Full Length Human GAD2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 65.2 kDa
• AA Sequence: MASPGSGFWSFGSEDGSGDSENPGT ARAWCQVAQKFTGGIGNKLCALLYG DAEKPAESGGSQPPRAAARK AACACDQKPCSCSKVDVNYAFLHAT DLLPACDGERPTLAFLQDVMNILLQ YVVKSFDRSTKVIDFHYPNE LLQEYNWELADQPQNLEEILMHCQT TLKYAIKTGHPRYFNQLSTGLDMVG LAADWLTSTANTNMFTYEIA PVFVLLEYVTLKKMREIIGWPGGSG DGIFSPGGAISNMYAMMIARFKMFP EVKEKGMAALPRLIAFTSEH SHFSLKKGAAALGIGTDSVILIKCD ERGKMIPSDLERRILEAKQKGFVPF LVSATAGTTVYGAFDPLLAV ADICKKYKIWMHVDAAWGGGLLMSR KHKWKLSGVERANSVTWNPHKMMGV PLQCSALLVREEGLMQNCNQ MHASYLFQQDKHYDLSYDTGDKALQ CGRHVDVFKLWLMWRAKGTTGFEAH VDKCLELAEYLYNIIKNREG YEMVFDGKPQHTNVCFWYIPPSLRT LEDNEERMSRLSKVAPVIKARMMEY GTTMVSYQPLGDKVNFFRMV
  ISNPAATHQDIDFLIEEIERLGQDL SGPTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Taurine and hypotaurine metabolism, Type I diabetes mellitus

Gene Information

• Gene Name: GAD2 glutamate decarboxylase 2 [ Homo sapiens (human) ]
• Official Symbol: GAD2
• Synonyms: GAD65
• Gene ID: 2572
• mRNA Refseq: NM_001134366.2
• Protein Refseq: NP_001127838.1
• MIM: 138275
• UniProt ID: Q05329