Recombinant Full Length Human GBE1 Protein, C-Flag-tagged

• Cat.No.: GBE1-2146HFL
• Product Overview: Recombinant Full Length Human GBE1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 80.3 kDa
• AA Sequence: MAAPMTPAARPEDYEAALNAALADV PELARLLEIDPYLKPYAVDFQRRYK QFSQILKNIGENEGGIDKFS RGYESFGVHRCADGGLYCKEWAPGA EGVFLTGDFNGWNPFSYPYKKLDYG KWELYIPPKQNKSVLVPHGS KLKVVITSKSGEILYRISPWAKYVV REGDNVNYDWIHWDPEHSYEFKHSR PKKPRSLRIYESHVGISSHE GKVASYKHFTCNVLPRIKGLGYNCI QLMAIMEHAYYASFGYQITSFFAAS SRYGSPEELQELVDTAHSMG IIVLLDVVHSHASKNSADGLNMFDG TDSCYFHSGPRGTHDLWDSRLFAYS SWEVLRFLLSNIRWWLEEYR FDGFRFDGVTSMLYHHHGVGQGFSG DYSEYFGLQVDEDALTYLMLANHLV HTLCPDSITIAEDVSGMPAL CSPISQGGGGFDYRLAMAIPDKWIQ LLKEFKDEDWNMGDIVYTLTNRRYL EKCIAYAESHDQALVGDKSL AFWLMDAEMYTNMSVLTPFTPVIDR GIQLHKMIRLITHGLGGEGYLNFMG NEFGHPEWLDFPRKGNNESY HYARRQFHLTDDDLLRYKFLNNFDR DMNRLEERYGWLAAPQAYVSEKHEG NKIIAFERAGLLFIFNFHPS KSYTDYRVGTALPGKFKIVLDSDAA EYGGHQRLDHSTDFFSEAFEHNGRP YSLLVYIPSRVALILQNVDL PN TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism

Gene Information

• Gene Name: GBE1 1,4-alpha-glucan branching enzyme 1 [ Homo sapiens (human) ]
• Official Symbol: GBE1
• Synonyms: GBE; APBD; GSD4
• Gene ID: 2632
• mRNA Refseq: NM_000158.4
• Protein Refseq: NP_000149.4
• MIM: 607839
• UniProt ID: Q04446