Recombinant Full Length Human GCK Protein, C-Flag-tagged

• Cat.No.: GCK-1984HFL
• Product Overview: Recombinant Full Length Human GCK Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 52 kDa
• AA Sequence: MLDDRARMEAAKKEKVEQILAEFQL QEEDLKKVMRRMQKEMDRGLRLETH EEASVKMLPTYVRSTPEGSE VGDFLSLDLGGTNFRVMLVKVGEGE EGQWSVKTKHQMYSIPEDAMTGTAE MLFDYISECISDFLDKHQMK HKKLPLGFTFSFPVRHEDIDKGILL NWTKGFKASGAEGNNVVGLLRDAIK RRGDFEMDVVAMVNDTVATM ISCYYEDHQCEVGMIVGTGCNACYM EEMQNVELVEGDEGRMCVNTEWGAF GDSGELDEFLLEYDRLVDES SANPGQQLYEKLIGGKYMGELVRLV LLRLVDENLLFHGEASEQLRTRGAF ETRFVSQVESDTGDRKQIYN ILSTLGLRPSTTDCDIVRRACESVS TRAAHMCSAGLAGVINRMRESRSED VMRITVGVDGSVYKLHPSFK ERFHASVRRLTPSCEITFIESEEGS GRGAALVSAVACKKACMLGQ TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus

Gene Information

• Gene Name: GCK glucokinase [ Homo sapiens (human) ]
• Official Symbol: GCK
• Synonyms: GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; PNDM1; FGQTL3
• Gene ID: 2645
• mRNA Refseq: NM_000162.5
• Protein Refseq: NP_000153.1
• MIM: 138079
• UniProt ID: P35557