Recombinant Full Length Human GFM1 Protein, C-Flag-tagged

• Cat.No.: GFM1-1770HFL
• Product Overview: Recombinant Full Length Human GFM1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 83.3 kDa
• AA Sequence: MRLLGAAAVAALGRGRAPASLGWQR KQVNWKACRWSSSGVIPNEKIRNIG ISAHIDSGKTTLTERVLYYT GRIAKMHEVKGKDGVGAVMDSMELE RQRGITIQSAATYTMWKDVNINIID TPGHVDFTIEVERALRVLDG AVLVLCAVGGVQCQTMTVNRQMKRY NVPFLTFINKLDRMGSNPARALQQM RSKLNHNAAFMQIPMGLEGN FKGIVDLIEERAIYFDGDFGQIVRY GEIPAELRAAATDHRQELIECVANS DEQLGEMFLEEKIPSISDLK LAIRRATLKRSFTPVFLGSALKNKG VQPLLDAVLEYLPNPSEVQNYAILN KEDDSKEKTKILMNSSRDNS HPFVGLAFKLEVGRFGQLTYVRSYQ GELKKGDTIYNTRTRKKVRLQRLAR MHADMMEDVEEVYAGDICAL FGIDCASGDTFTDKANSGLSMESIH VPDPVISIAMKPSNKNDLEKFSKGI GRFTREDPTFKVYFDTENKE TVISGMGELHLEIYAQRLEREYGCP CITGKPKVAFRETITAPVPFDFTHK KQSGGAGQYGKVIGVLEPLD PEDYTKLEFSDETFGSNIPKQFVPA VEKGFLDACEKGPLSGHKLSGLRFV LQDGAHHMVDSNEISFIRAG EGALKQALANATLCILEPIMAVEVV APNEFQGQVIAGINRRHGVITGQDG VEDYFTLYADVPLNDMFGYS TELRSCTEGKGEYTMEYSRYQPCLP STQEDVINKYLEATGQLPVKKGKAK N SGPTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: GFM1 G elongation factor mitochondrial 1 [ Homo sapiens (human) ]
• Official Symbol: GFM1
• Synonyms: EFG; GFM; EFG1; EFGM; EGF1; hEFG1; COXPD1; mtEF-G1
• Gene ID: 85476
• mRNA Refseq: NM_024996.7
• Protein Refseq: NP_079272.4
• MIM: 606639
• UniProt ID: Q96RP9