Recombinant Full Length Human HK1 Protein, C-Flag-tagged

• Cat.No.: HK1-832HFL
• Product Overview: Recombinant Full Length Human HK1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 102.3 kDa
• AA Sequence: MIAAQLLAYYFTELKDDQVKKIDKY LYAMRLSDETLIDIMTRFRKEMKNG LSRDFNPTATVKMLPTFVRS IPDGSEKGDFIALDLGGSSFRILRV QVNHEKNQNVHMESEVYDTPENIVH GSGSQLFDHVAECLGDFMEK RKIKDKKLPVGFTFSFPCQQSKIDE AILITWTKRFKASGVEGADVVKLLN KAIKKRGDYDANIVAVVNDT VGTMMTCGYDDQHCEVGLIIGTGTN ACYMEELRHIDLVEGDEGRMCINTE WGAFGDDGSLEDIRTEFDRE IDRGSLNPGKQLFEKMVSGMYLGEL VRLILVKMAKEGLLFEGRITPELLT RGKFNTSDVSAIEKNKEGLH NAKEILTRLGVEPSDDDCVSVQHVC TIVSFRSANLVAATLGAILNRLRDN KGTPRLRTTVGVDGSLYKTH PQYSRRFHKTLRRLVPDSDVRFLLS ESGSGKGAAMVTAVAYRLAEQHRQI EETLAHFHLTKDMLLEVKKR MRAEMELGLRKQTHNNAVVKMLPSF VRRTPDGTENGDFLALDLGGTNFRV LLVKIRSGKKRTVEMHNKIY AIPIEIMQGTGEELFDHIVSCISDF LDYMGIKGPRMPLGFTFSFPCQQTS LDAGILITWTKGFKATDCVG HDVVTLLRDAIKRREEFDLDVVAVV NDTVGTMMTCAYEEPTCEVGLIVGT GSNACYMEEMKNVEMVEGDQ GQMCINMEWGAFGDNGCLDDIRTHY DRLVDEYSLNAGKQRYEKMISGMYL GEIVRNILIDFTKKGFLFRG QISETLKTRGIFETKFLSQIESDRL ALLQVRAILQQLGLNSTCDDSILVK TVCGVVSRRAAQLCGAGMAA VVDKIRENRGLDRLNVTVGVDGTLY KLHPHFSRIMHQTVKELSPKCNVSF LLSEDGSGKGAALITAVGVR
  LRTEASSTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus

Gene Information

• Gene Name: HK1 hexokinase 1 [ Homo sapiens (human) ]
• Official Symbol: HK1
• Synonyms: HK; HKD; HKI; HXK1; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; NEDVIBA; hexokinase
• Gene ID: 3098
• mRNA Refseq: NM_000188.3
• Protein Refseq: NP_000179.2
• MIM: 142600
• UniProt ID: P19367