| Description : |
The authors of PMID:20797690 cloned this gene while searching for genes in a region of chromosome 10 linked to primary hyperoxalurea type III. They noted that even though the encoded protein has been described as a mitochondrial dihydrodipicolinate synthase-like enzyme, it shares little homology with E. coli dihydrodipicolinate synthase (Dhdps), particularly in the putative substrate-binding region. Moreover, neither lysine biosynthesis nor sialic acid metabolism, for which Dhdps is responsible, occurs in vertebrate mitochondria. They propose that this gene encodes mitochondrial 4-hydroxyl-2-oxoglutarate aldolase (EC 4.1.3.16), which catalyzes the final step in the metabolic pathway of hydroxyproline, releasing glyoxylate and pyruvate. This gene is predominantly expressed in the liver and kidney, and mutations in this gene are found in patients with primary hyperoxalurea type III. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| Source : |
In Vitro Cell Free System |
| Species : |
Human |
| Tag : |
GST |
| Form : |
50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
| Molecular Mass : |
61.6 kDa |
| Protein Length : |
327 amino acids |
| AA Sequence : |
MLGPQVWSSVRQGLSRSLSRNVGVW ASGEGKKVDIAGIYPPVTTPFTATA EVDYGKLEENLHKLGTFPFRGFVVQ GSNGEFPFLTSSERLEVVSRVRQAM PKNRLLLAGSGCESTQATVEMTVSM AQVGADAAMVVTPCYYRGRMSSAAL IHHYTKVADLSPIPVVLYSVPANTG LDLPVDAVVTLSQHPNIVGMKDSGG DVTRIGLIVHKTRKQDFQVLAGSAG FLMASYALGAVGGVCALANVLGAQV CQLERLCCTGQWEDAQKLQHRLIEP NAAVTRRFGIPGLKKIMDWFGYYGG PCRAPLQELSPAEEEALRMDFTSNG WL |
| Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Storage Buffer : |
50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
