Recombinant Full Length Human HSPA8 Protein, C-Flag-tagged

• Cat.No.: HSPA8-342HFL
• Product Overview: Recombinant Full Length Human HSPA8 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 70.7 kDa
• AA Sequence: MSKGPAVGIDLGTTYSCVGVFQHGK VEIIANDQGNRTTPSYVAFTDTERL IGDAAKNQVAMNPTNTVFDA KRLIGRRFDDAVVQSDMKHWPFMVV NDAGRPKVQVEYKGETKSFYPEEVS SMVLTKMKEIAEAYLGKTVT NAVVTVPAYFNDSQRQATKDAGTIA GLNVLRIINEPTAAAIAYGLDKKVG AERNVLIFDLGGGTFDVSIL TIEDGIFEVKSTAGDTHLGGEDFDN RMVNHFIAEFKRKHKKDISENKRAV RRLRTACERAKRTLSSSTQA SIEIDSLYEGIDFYTSITRARFEEL NADLFRGTLDPVEKALRDAKLDKSQ IHDIVLVGGSTRIPKIQKLL QDFFNGKELNKSINPDEAVAYGAAV QAAILSGDKSENVQDLLLLDVTPLS LGIETAGGVMTVLIKRNTTI PTKQTQTFTTYSDNQPGVLIQVYEG ERAMTKDNNLLGKFELTGIPPAPRG VPQIEVTFDIDANGILNVSA VDKSTGKENKITITNDKGRLSKEDI ERMVQEAEKYKAEDEKQRDKVSSKN SLESYAFNMKATVEDEKLQG KINDEDKQKILDKCNEIINWLDKNQ TAEKEEFEHQQKELEKVCNPIITKL YQSAGGMPGGMPGGFPGGGA
  PPSGGASSGPTIEEVDTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome

Gene Information

• Gene Name: HSPA8 heat shock protein family A (Hsp70) member 8 [ Homo sapiens (human) ]
• Official Symbol: HSPA8
• Synonyms: LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
• Gene ID: 3312
• mRNA Refseq: NM_006597.6
• Protein Refseq: NP_006588.1
• MIM: 600816
• UniProt ID: P11142

Reviews

12/01/2021

The lab product's precision and quality are noteworthy.

10/25/2020

The customer service team was accommodating, and the delivery exceeded my expectations.

05/10/2018

This lab product is synonymous with excellence.

Q&As

In the context of cellular stress, how does HSPA8 contribute to the heat shock response, and what downstream signaling pathways does it modulate to promote cell survival?

HSPA8 is a central player in the heat shock response, assisting in the refolding of denatured proteins. It modulates downstream pathways such as HSF1 activation, orchestrating a cellular survival response.

Can you discuss the involvement of HSPA8 in neurodegenerative diseases, and how does its chaperone activity influence protein aggregation and neuronal dysfunction?

HSPA8 plays a role in neurodegenerative diseases, mitigating protein aggregation and neuronal dysfunction. Its chaperone activity is crucial for maintaining protein homeostasis in the nervous system.

How does HSPA8 participate in the regulation of apoptosis, and what anti-apoptotic mechanisms does it employ to maintain cellular viability under stress conditions?

HSPA8 exhibits anti-apoptotic functions by interacting with pro-apoptotic proteins and preventing their activation. Its role in apoptosis regulation contributes to cell survival during stress.

What is the significance of HSPA8 in autophagy, and how does it influence the selective degradation of misfolded proteins and damaged organelles?

HSPA8 is involved in autophagy, facilitating the clearance of misfolded proteins and damaged organelles. It interacts with autophagy receptors, promoting their recognition by autophagosomes.

What are the mechanisms by which HSPA8 regulates the immune response, particularly its involvement in antigen presentation and the modulation of immune cell functions?

HSPA8 participates in antigen presentation and modulates immune cell functions. It chaperones antigens for presentation on major histocompatibility complexes, influencing immune recognition and response.