Recombinant Full Length Human HTT Protein, C-Flag-tagged

• Cat.No.: HTT-20HFL
• Product Overview: Recombinant Full Length Human HTT Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range of trinucleotide repeats (9-35) has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 347.7 kDa
• AA Sequence: MATLEKLMKAFESLKSFQQQQQQQQ QQQQQQQQQQQQQQQPPPPPPPPPP PQLPQPPPQAQPLLPQPQPP PPPPPPPPGPAVAEEPLHRPKKELS ATKKDRVNHCLTICENIVAQSVRNS PEFQKLLGIAMELFLLCSDD AESDVRMVADECLNKVIKALMDSNL PRLQLELYKEIKKNGAPRSLRAALW RFAELAHLVRPQKCRPYLVN LLPCLTRTSKRPEESVQETLAAAVP KIMASFGNFANDNEIKVLLKAFIAN LKSSSPTIRRTAAGSAVSIC QHSRRTQYFYSWLLNVLLGLLVPVE DEHSTLLILGVLLTLRYLVPLLQQQ VKDTSLKGSFGVTRKEMEVS PSAEQLVQVYELTLHHTQHQDHNVV TGALELLQQLFRTPPPELLQTLTAV GGIGQLTAAKEESGGRSRSG SIVELIAGGGSSCSPVLSRKQKGKV LLGEEEALEDDSESRSDVSSSALTA SVKDEISGELAASSGVSTPG SAGHDIITEQPRSQHTLQADSVDLA SCDLTSSATDGDEEDILSHSSSQVS AVPSDPAMDLNDGTQASSPI SDSSQTTTEGPDSAVTPSDSSEIVL DGTDNQYLGLQIGQPQDEDEEATGI LPDEASEAFRNSSMALQQAH LLKNMSHCRQPSDSSVDKFVLRDEA TEPGDQENKPCRIKGDIGQSTDDDS APLVHCVRLLSASFLLTGGK NVLVPDRDVRVSVKALALSCVGAAV ALHPESFFSKLYKVPLDTTEYPEEQ YVSDILNYIDHGDPQVRGAT AILCGTLICSILSRSRFHVGDWMGT IRTLTGNTFSLADCIPLLRKTLKDE SSVTCKLACTAVRNCVMSLC SSSYSELGLQLIIDVLTLRNSSYWL VRTELLETLAEIDFRLVSFLEAKAE NLHRGAHHYTGLLKLQERVL NNVVIHLLGDEDPRVRHVAAASLIR LVPKLFYKCDQGQADPVVAVARDQS SVYLKLLMHETQPPSHFSVS TITRIYRGYNLLPSITDVTMENNLS RVIAAVSHELITSTTRALTFGCCEA LCLLSTAFPVCIWSLGWHCG VPPLSASDESRKSCTVGMATMILTL LSSAWFPLDLSAHQDALILAGNLLA ASAPKSLRSSWASEEEANPA ATKQEEVWPALGDRALVPMVEQLFS HLLKVINICAHVLDDVAPGPAIKAA LPSLTNPPSLSPIRRKGKEK EPGEQASVPLSPKKGSEASAASRQS DTSGPVTTSKSSSLGSFYHLPSYLK LHDVLKATHANYKVTLDLQN STEKFGGFLRSALDVLSQILELATL QDIGKCVEEILGYLKSCFSREPMMA TVCVQQLLKTLFGTNLASQF DGLSSNPSKSQGRAQRLGSSSVRPG LYHYCFMAPYTHFTQALADASLRNM VQAEQENDTSGWFDVLQKVS TQLKTNLTSVTKNRADKNAIHNHIR LFEPLVIKALKQYTTTTCVQLQKQV LDLLAQLVQLRVNYCLLDSD QVFIGFVLKQFEYIEVGQFRESEAI IPNIFFFLVLLSYERYHSKQIIGIP KIIQLCDGIMASGRKAVTHA IPALQPIVHDLFVLRGTNKADAGKE LETQKEVVVSMLLRLIQYHQVLEMF ILVLQQCHKENEDKWKRLSR QIADIILPMLAKQQMHIDSHEALGV LNTLFEILAPSSLRPVDMLLRSMFV TPNTMASVSTVQLWISGILA ILRVLISQSTEDIVLSRIQELSFSP YLISCTVINRLRDGDSTSTLEEHSE GKQIKNLPEETFSRFLLQLV GILLEDIVTKQLKVEMSEQQHTFYC QELGTLLMCLIHIFKSGMFRRITAA ATRLFRSDGCGGSFYTLDSL NLRARSMITTHPALVLLWCQILLLV NHTDYRWWAEVQQTPKRHSLSSTKL LSPQMSGEEEDSDLAAKLGM CNREIVRRGALILFCDYVCQNLHDS EHLTWLIVNHIQDLISLSHEPPVQD FISAVHRNSAASGLFIQAIQ SRCENLSTPTMLKKTLQCLEGIHLS QSGAVLTLYVDRLLCTPFRVLARMV DILACRRVEMLLAANLQSSM AQLPMEELNRIQEYLQSSGLAQRHQ RLYSLLDRFRLSTMQDSLSPSPPVS SHPLDGDGHVSLETVSPDKD WYVHLVKSQCWTRSDSALLEGAELV NRIPAEDMNAFMMNSEFNLSLLAPC LSLGMSEISGGQKSALFEAA REVTLARVSGTVQQLPAVHHVFQPE LPAEPAAYWSKLNDLFGDAALYQSL PTLARALAQYLVVVSKLPSH LHLPPEKEKDIVKFVVATLEALSWH LIHEQIPLSLDLQAGLDCCCLALQL PGLWSVVSSTEFVTHACSLI YCVHFILEAVAVQPGEQLLSPERRT NTPKAISEEEEEVDPNTQNPKYITA ACEMVAEMVESLQSVLALGH KRNSGVPAFLTPLLRNIIISLARLP LVNSYTRVPPLVWKLGWSPKPGGDF GTAFPEIPVEFLQEKEVFKE FIYRINTLGWTSRTQFEETWATLLG VLVTQPLVMEQEESPPEEDTERTQI NVLAVQAITSLVLSAMTVPV AGNPAVSCLEQQPRNKPLKALDTRF GRKLSIIRGIVEQEIQAMVSKRENI ATHHLYQAWDPVPSLSPATT GALISHEKLLLQINPERELGSMSYK LGQVSIHSVWLGNSITPLREEEWDE EEEEEADAPAPSSPPTSPVN SRKHRAGVDIHSCSQFLLELYSRWI LPSSSARRTPAILISEVVRSLLVVS DLFTERNQFELMYVTLTELR RVHPSEDEILAQYLVPATCKAAAVL GMDKAVAEPVSRLLESTLRSSHLPS RVGALHGVLYVLECDLLDDT AKQLIPVISDYLLSNLKGIAHCVNI HSQQHVLVMCATAFYLIENYPLDVG PEFSASIIQMCGVMLSGSEE STPSIIYHCALRGLERLLLSEQLSR LDAESLVKLSVDRVNVHSPHRAMAA LGLMLTCMYTGKEKVSPGRT SDPNPAAPDSESVIVAMERVSVLFD RIRKGFPCEARVVARILPQFLDDFF PPQDIMNKVIGEFLSNQQPY PQFMATVVYKVFQTLHSTGQSSMVR DWVMLSLSNFTQRAPVAMATWSLSC FFVSASTSPWVAAILPHVIS
  RMGKLEQVDVNLFCLVATDFYRHQI EEELDRRAFQSVLEVVAAPGSPYHR LLTCLRNVHKVTTCTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease

Gene Information

• Gene Name: HTT huntingtin [ Homo sapiens (human) ]
• Official Symbol: HTT
• Synonyms: HD; IT15; LOMARS
• Gene ID: 3064
• mRNA Refseq: NM_002111.8
• Protein Refseq: NP_002102.4
• MIM: 613004
• UniProt ID: P42858

Reviews

06/24/2021

This protein synthesis process is efficient and reliable, reducing experimental time and cost.

02/20/2021

This product has strict quality control and complies with relevant industry standards.

09/08/2020

When experiments were performed with this protein, the sensitivity and accuracy of the experiments were significantly improved.

Q&As

What diseases is HTT associated with?

HTT is associated with neurodegenerative diseases such as Huntington's disease, Parkinson's disease, and Alzheimer's disease.

How can animal models be used to study the function and disease mechanism of HTT protein?

Animal models carrying human mutant genes can be constructed using transgenic or gene editing technology to study the role of HTT protein in vivo and disease mechanisms. These animal models can be used to study the effects of HTT proteins on neuronal function, synaptic transmission, behavior, and more, as well as to evaluate the effects of potential therapeutic strategies.

What are the therapeutic strategies for HTT protein?

Therapeutic strategies for HTT protein include inhibiting its expression, reducing its activity, or changing its modification state. A number of small molecule inhibitors and gene therapy strategies targeting HTT proteins are currently being studied.

Is there a challenge in HTT protein research?

There are still some challenges in the research of HTT proteins, such as the need to better understand their structure and functional relationships, and develop more effective drugs or treatments. In addition, due to the complexity and individual differences of neurodegenerative diseases, therapeutic strategies targeting HTT proteins also need to be adapted and optimized according to individual conditions.

How does HTT protein interact with other proteins or signaling pathways?

HTT protein can interact with other proteins and signaling pathways. For example, HTT proteins can interact with transcription factors to affect gene transcription, and can also interact with signaling pathways such as MAPK (mitogen-activated protein kinase) to participate in cell proliferation and apoptosis.

How can HTT protein be used for disease diagnosis?

By measuring the expression level and modification status of HTT protein, it can be used to diagnose diseases such as Huntington's disease. At present, there are some detection methods for HTT protein, such as western blotting, immunohistochemistry, etc.