Recombinant Full Length Human ITK Protein, C-Flag-tagged

• Cat.No.: ITK-1301HFL
• Product Overview: Recombinant Full Length Human ITK Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 71.7 kDa
• AA Sequence: MNNFILLEEQLIKKSQQKRRTSPSN FKVRFFVLTKASLAYFEDRHGKKRT LKGSIELSRIKCVEIVKSDI SIPCHYKYPFQVVHDNYLLYVFAPD RESRQRWVLALKEETRNNNSLVPKY HPNFWMDGKWRCCSQLEKLA TGCAQYDPTKNASKKPLPPTPEDNR RPLWEPEETVVIALYDYQTNDPQEL ALRRNEEYCLLDSSEIHWWR VQDRNGHEGYVPSSYLVEKSPNNLE TYEWYNKSISRDKAEKLLLDTGKEG AFMVRDSRTAGTYTVSVFTK AVVSENNPCIKHYHIKETNDNPKRY YVAEKYVFDSIPLLINYHQHNGGGL VTRLRYPVCFGRQKAPVTAG LRYGKWVIDPSELTFVQEIGSGQFG LVHLGYWLNKDKVAIKTIREGAMSE EDFIEEAEVMMKLSHPKLVQ LYGVCLEQAPICLVFEFMEHGCLSD YLRTQRGLFAAETLLGMCLDVCEGM AYLEEACVIHRDLAARNCLV GENQVIKVSDFGMTRFVLDDQYTSS TGTKFPVKWASPEVFSFSRYSSKSD VWSFGVLMWEVFSEGKIPYE
  NRSNSEVVEDISTGFRLYKPRLAST HVYQIMNHCWKERPEDRPAFSRLLR QLAEIAESGLTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Leukocyte transendothelial migration, T cell receptor signaling pathway

Gene Information

• Gene Name: ITK IL2 inducible T cell kinase [ Homo sapiens (human) ]
• Official Symbol: ITK
• Synonyms: EMT; LYK; LPFS1; PSCTK2
• Gene ID: 3702
• mRNA Refseq: NM_005546.4
• Protein Refseq: NP_005537.3
• MIM: 186973
• UniProt ID: Q08881