Recombinant Full Length Human KLHL3 Protein, C-Flag-tagged

• Cat.No.: KLHL3-1136HFL
• Product Overview: Recombinant Full Length Human KLHL3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 64.8 kDa
• AA Sequence: MEGESVKLSSQTLIQAGDDEKNQRT ITVNPAHMGKAFKVMNELRSKQLLC DVMIVAEDVEIEAHRVVLAA CSPYFCAMFTGDMSESKAKKIEIKD VDGQTLSKLIDYIYTAEIEVTEENV QVLLPAASLLQLMDVRQNCC DFLQSQLHPTNCLGIRAFADVHTCT DLLQQANAYAEQHFPEVMLGEEFLS LSLDQVCSLISSDKLTVSSE EKVFEAVISWINYEKETRLEHMAKL MEHVRLPLLPRDYLVQTVEEEALIK NNNTCKDFLIEAMKYHLLPL DQRLLIKNPRTKPRTPVSLPKVMIV VGGQAPKAIRSVECYDFEEDRWDQI AELPSRRCRAGVVFMAGHVY AVGGFNGSLRVRTVDVYDGVKDQWT SIASMQERRSTLGAAVLNDLLYAVG GFDGSTGLASVEAYSYKTNE WFFVAPMNTRRSSVGVGVVEGKLYA VGGYDGASRQCLSTVEQYNPATNEW IYVADMSTRRSGAGVGVLSG QLYATGGHDGPLVRKSVEVYDPGTN TWKQVADMNMCRRNAGVCAVNGLLY VVGGDDGSCNLASVEYYNPV
  TDKWTLLPTNMSTGRSYAGVAVIHK SLTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: KLHL3 kelch like family member 3 [ Homo sapiens (human) ]
• Official Symbol: KLHL3
• Synonyms: PHA2D
• Gene ID: 26249
• mRNA Refseq: NM_017415.3
• Protein Refseq: NP_059111.2
• MIM: 605775
• UniProt ID: Q9UH77