Recombinant Full Length Human KPNA4 Protein, C-Flag-tagged

• Cat.No.: KPNA4-2192HFL
• Product Overview: Recombinant Full Length Human KPNA4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 57.7 kDa
• AA Sequence: MADNEKLDNQRLKNFKNKGRDLETM RRQRNEVVVELRKNKRDEHLLKRRN VPHEDICEDSDIDGDYRVQN TSLEAIVQNASSDNQGIQLSAVQAA RKLLSSDRNPPIDDLIKSGILPILV HCLERDDNPSLQFEAAWALT NIASGTSEQTQAVVQSNAVPLFLRL LHSPHQNVCEQAVWALGNIIGDGPQ CRDYVISLGVVKPLLSFISP SIPITFLRNVTWVMVNLCRHKDPPP PMETIQEILPALCVLIHHTDVNILV DTVWALSYLTDAGNEQIQMV IDSGIVPHLVPLLSHQEVKVQTAAL RAVGNIVTGTDEQTQVVLNCDALSH FPALLTHPKEKINKEAVWFL SNITAGNQQQVQAVIDANLVPMIIH LLDKGDFGTQKEAAWAISNLTISGR KDQVAYLIQQNVIPPFCNLL TVKDAQVVQVVLDGLSNILKMAEDE AETIGNLIEECGGLEKIEQLQNHEN EDIYKLAYEIIDQFFSSDDI DEDPSLVPEAIQGGTFGFNSSANVP TEGFQF TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: KPNA4 karyopherin subunit alpha 4 [ Homo sapiens (human) ]
• Official Symbol: KPNA4
• Synonyms: QIP1; SRP3; IPOA3
• Gene ID: 3840
• mRNA Refseq: NM_002268.5
• Protein Refseq: NP_002259.1
• MIM: 602970
• UniProt ID: O00629