Recombinant Full Length Human MAP2K4 Protein, C-Flag-tagged

• Cat.No.: MAP2K4-1091HFL
• Product Overview: Recombinant Full Length Human MAP2K4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 44.1 kDa
• AA Sequence: MAAPSPSGGGGSGGGRGSGTPGPVG SPAPGHPAVSSMQGKRKALKLNFAN PPFKSTARFTLNPNPTGVQN PHIERLRTHSIESSGKLKISPEQHW DFTAEDLKDLGEIGRGAYGSVNKMV HKPSGQIMAVKRIRSTVDEK EQKQLLMDLDVVMRSSDCPYIVQFY GALFREGDCWICMELMSTSFDKFYK YVYSVLDDVIPEEILGKITL ATVKALNHLKENLKIIHRDIKPSNI LLDRSGNIKLCDFGISGQLVDSIAK TRDAGCRPYMAPERIDPSAS RQGYDVRSDVWSLGITLYELATGRF PYPKWNSVFDQLTQVVKGDPPQLSN SEEREFSPSFINFVNLCLTK
  DESKRPKYKELLKHPFILMYEERAV EVACYVCKILDQMPATPSSPMYVDT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway

Gene Information

• Gene Name: MAP2K4 mitogen-activated protein kinase kinase 4 [ Homo sapiens (human) ]
• Official Symbol: MAP2K4
• Synonyms: JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
• Gene ID: 6416
• mRNA Refseq: NM_003010.4
• Protein Refseq: NP_003001.1
• MIM: 601335
• UniProt ID: P45985

Reviews

10/25/2021

Good value, high quality.

04/03/2021

Pure product, no issues.

03/13/2021

Reliable, worked as described.

Q&As

Are there existing or potential therapeutic strategies targeting MAP2K4?

Targeting MAP2K4 may provide therapeutic benefits in cancer, modulating cell survival and response to stress.

What role does MAP2K4 play in cellular stress response?

MAP2K4 helps cells respond to stress, modulating survival and death pathways.

How is MAP2K4 implicated in cancer development and progression?

Dysregulation of MAP2K4 has been linked to cancer, affecting cell growth and resistance to apoptosis.

How does MAP2K4 contribute to the JNK and p38 MAPK pathways?

It phosphorylates and activates JNK and p38, transmitting signals in response to stress.

How is the activity of MAP2K4 regulated within cells?

Its activity is regulated by phosphorylation and interaction with other signaling proteins.

In which cellular processes is MAP2K4 involved?

MAP2K4 is involved in processes like cell growth, apoptosis, and stress response.

What is the primary biological function of MAP2K4 in cellular signaling?

MAP2K4 is a kinase that plays a critical role in activating the JNK and p38 MAPK signaling pathways.