Recombinant Full Length Human MAP3K1 Protein, C-Flag-tagged

• Cat.No.: MAP3K1-1515HFL
• Product Overview: Recombinant Full Length Human MAP3K1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 164.3 kDa
• AA Sequence: MAAAAGNRASSSGFPGARATSPEAG GGGGALKASSAPAAAAGLLREAGSG GRERADWRRRQLRKVRSVEL DQLPEQPLFLAASPPASSTSPSPEP ADAAGSGTGFQPVAVPPPHGAASRG GAHLTESVAAPDSGASSPAA AEPGEKRAPAAEPSPAAAPAGREME NKETLKGLHKMDDRPEERMIREKLK ATCMPAWKHEWLERRNRRGP VVVKPIPVKGDGSEMNHLAAESPGE VQASAASPASKGRRSPSPGNSPSGR TVKSESPGVRRKRVSPVPFQ SGRITPPRRAPSPDGFSPYSPEETN RRVNKVMRARLYLLQQIGPNSFLIG GDSPDNKYRVFIGPQNCSCA RGTFCIHLLFVMLRVFQLEPSDPML WRKTLKNFEVESLFQKYHSRRSSRI KAPSRNTIQKFVSRMSNSHT LSSSSTSTSSSENSIKDEEEQMCPI CLLGMLDEESLTVCEDGCRNKLHHH CMSIWAEECRRNREPLICPL CRSKWRSHDFYSHELSSPVDSPSSL RAAQQQTVQQQPLAGSRRNQESNFN LTHYGTQQIPPAYKDLAEPW IQVFGMELVGCLFSRNWNVREMALR RLSHDVSGALLLANGESTGNSGGSS GSSPSGGATSGSSQTSISGD VVEACCSVLSMVCADPVYKVYVAAL KTLRAMLVYTPCHSLAERIKLQRLL QPVVDTILVKCADANSRTSQ LSISTLLELCKGQAGELAVGREILK AGSIGIGGVDYVLNCILGNQTESNN WQELLGRLCLIDRLLLEFPA EFYPHIVSTDVSQAEPVEIRYKKLL SLLTFALQSIDNSHSMVGKLSRRIY LSSARMVTTVPHVFSKLLEM LSVSSSTHFTRMRRRLMAIADEVEI AEAIQLGVEDTLDGQQDSFLQASVP NNYLETTENSSPECTVHLEK TGKGLCATKLSASSEDISERLASIS VGPSSSTTTTTTTTEQPKPMVQTKG RPHSQCLNSSPLSHHSQLMF PALSTPSSSTPSVPAGTATDVSKHR LQGFIPCRIPSASPQTQRKFSLQFH RNCPENKDSDKLSPVFTQSR PLPSSNIHRPKPSRPTPGNTSKQGD PSKNSMTLDLNSSSKCDDSFGCSSN SSNAVIPSDETVFTPVEEKC RLDVNTELNSSIEDLLEASMPSSDT TVTFKSEVAVLSPEKAENDDTYKDD VNHNQKCKEKMEAEEEEALA IAMAMSASQDALPIVPQLQVENGED IIIIQQDTPETLPGHTKAKQPYRED TEWLKGQQIGLGAFSSCYQA QDVGTGTLMAVKQVTYVRNTSSEQE EVVEALREEIRMMSHLNHPNIIRML GATCEKSNYNLFIEWMAGGS VAHLLSKYGAFKESVVINYTEQLLR GLSYLHENQIIHRDVKGANLLIDST GQRLRIADFGAAARLASKGT GAGEFQGQLLGTIAFMAPEVLRGQQ YGRSCDVWSVGCAIIEMACAKPPWN AEKHSNHLALIFKIASATTA
  PSIPSHLSPGLRDVALRCLELQPQD RPPSRELLKHPVFRTTWTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, RIG-I-like receptor signaling pathway, Ubiquitin mediated proteolysis

Gene Information

• Gene Name: MAP3K1 mitogen-activated protein kinase kinase kinase 1 [ Homo sapiens (human) ]
• Official Symbol: MAP3K1
• Synonyms: MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
• Gene ID: 4214
• mRNA Refseq: NM_005921.2
• Protein Refseq: NP_005912.1
• MIM: 600982
• UniProt ID: Q13233