Recombinant Full Length Human MAP3K5 Protein, C-Flag-tagged

• Cat.No.: MAP3K5-361HFL
• Product Overview: Recombinant Full Length Human MAP3K5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 154.4 kDa
• AA Sequence: MSTEADEGITFSVPPFAPSGFCTIP EGGICRRGGAAAVGEGEEHQLPPPP PGSFWNVESAAAPGIGCPAA TSSSSATRGRGSSVGGGSRRTTVAY VINEASQGQLVVAESEALQSLREAC ETVGATLETLHFGKLDFGET TVLDRFYNADIAVVEMSDAFRQPSL FYHLGVRESFSMANNIILYCDTNSD SLQSLKEIICQKNTMCTGNY TFVPYMITPHNKVYCCDSSFMKGLT ELMQPNFELLLGPICLPLVDRFIQL LKVAQASSSQYFRESILNDI RKARNLYTGKELAAELARIRQRVDN IEVLTADIVINLLLSYRDIQDYDSI VKLVETLEKLPTFDLASHHH VKFHYAFALNRRNLPGDRAKALDIM IPMVQSEGQVASDMYCLVGRIYKDM FLDSNFTDTESRDHGASWFK KAFESEPTLQSGINYAVLLLAAGHQ FESSFELRKVGVKLSSLLGKKGNLE KLQSYWEVGFFLGASVLAND HMRVIQASEKLFKLKTPAWYLKSIV ETILIYKHFVKLTTEQPVAKQELVD FWMDFLVEATKTDVTVVRFP VLILEPTKIYQPSYLSINNEVEEKT ISIWHVLPDDKKGIHEWNFSASSVR GVSISKFEERCCFLYVLHNS DDFQIYFCTELHCKKFFEMVNTITE EKGRSTEEGDCESDLLEYDYEYDEN GDRVVLGKGTYGIVYAGRDL SNQVRIAIKEIPERDSRYSQPLHEE IALHKHLKHKNIVQYLGSFSENGFI KIFMEQVPGGSLSALLRSKW GPLKDNEQTIGFYTKQILEGLKYLH DNQIVHRDIKGDNVLINTYSGVLKI SDFGTSKRLAGINPCTETFT GTLQYMAPEIIDKGPRGYGKAADIW SLGCTIIEMATGKPPFYELGEPQAA MFKVGMFKVHPEIPESMSAE AKAFILKCFEPDPDKRACANDLLVD EFLKVSSKKKKTQPKLSALSAGSNE YLRSISLPVPVLVEDTSSSS EYGSVSPDTELKVDPFSFKTRAKSC GERDVKGIRTLFLGIPDENFEDHSA PPSPEEKDSGFFMLRKDSER RATLHRILTEDQDKIVRNLMESLAQ GAEEPKLKWEHITTLIASLREFVRS TDRKIIATTLSKLKLELDFD SHGISQVQVVLFGFQDAVNKVLRNH NIKPHWMFALDSIIRKAVQTAITIL VPELRPHFSLASESDTADQE DLDVEDDHEEQPSNQTVRRPQAVIE DAVATSGVSTLSSTVSHDSQSAHRS LNVQLGRMKIETNRLLEELV RKEKELQALLHRAIEEKDQEIKHLK LKSQPIEIPELPVFHLNSSGTNTED SELTDWLRVNGADEDTISRF
  LAEDYTLLDVLYYVTRDDLKCLRLR GGMLCTLWKAIIDFRNKQTTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway, Neurotrophin signaling pathway

Gene Information

• Gene Name: MAP3K5 mitogen-activated protein kinase kinase kinase 5 [ Homo sapiens (human) ]
• Official Symbol: MAP3K5
• Synonyms: ASK1; MEKK5; MAPKKK5
• Gene ID: 4217
• mRNA Refseq: NM_005923.4
• Protein Refseq: NP_005914.1
• MIM: 602448
• UniProt ID: Q99683

Reviews

06/30/2020

The MAP3K5 protein has proven to be invaluable in protein electron microscopy structure analysis.

07/24/2018

Its ability to provide accurate and reliable results in this particular application makes it a preferred choice for researchers studying antigen-antibody interactions and immune responses.

12/01/2016

The MAP3K5 protein's sensitivity and specificity ensure precise measurements, contributing to the overall success of ELISA experiments.

Q&As

What other diseases or conditions involve MAP3K5?

MAP3K5 has been implicated in various diseases, including neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions.

How is MAP3K5 protein implicated in cancer?

Aberrant regulation of MAP3K5 has been associated with cancer development, as it can influence cell survival and death pathways.

Can MAP3K5 be used as a biomarker for certain diseases?

MAP3K5 expression levels and activity may serve as biomarkers for specific diseases, providing valuable information for diagnosis and prognosis.

Are there any clinical applications of MAP3K5 in cancer treatment?

Research suggests that targeting MAP3K5 could have therapeutic implications in cancer treatment, potentially through the modulation of apoptosis in cancer cells.

Are there ongoing clinical trials involving MAP3K5 inhibitors?

Investigational studies and clinical trials are underway to explore the efficacy of MAP3K5 inhibitors in different disease contexts, including cancer.