Recombinant Full Length Human MED25 Protein, GST-tagged

• Cat.No.: MED25-6245HF
• Product Overview: Human MED25 full-length ORF ( ACE87677.1, 1 a.a. - 747 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 108.57 kDa
• Protein Length: 747 amino acids
• AA Sequence: MVPGSEGPARAGSVVADVVFVIEGT ANLGPYFEGLRKHYLLPAIEYFNGG PPAETDFGGDYGGTQYSLVVFNTVD CAPESYVQCHAPTSSAYEFVTWLDG IKFMGGGGESCSLIAEGLSTALQLF DDFKKMREQIGQTHRVCLLICNSPP YLLPAVESTTYSGCTTENLVQQIGE RGIHFSIVSPRKLPALRLLFEKAAP PALLEPLQPPTDVSQDPRHMVLVRG LVLPVGGGSAPGPLQSKQPVPLPPA APSGATLSAAPQQPLPPVPPQYQVP GNLSAAQVAAQNAVEAAKNQKAGLG PRFSPITPLQQAAPGVGPPFSQAPA PQLPPGPPGAPKPPPASQPSLVSTV APGSGLAPTAQPGAPSMAGTVAPGG VSGPSPAQLGAPALGGQQSVSNKLL AWSGVLEWQEKPKPASVDANTKLTR SLPCQVYVNHGENLKTEQWPQKLIM QLIPQQLLTTLGPLFRNSRMVQFHF TNKDLESLKGLYRIMGNGFAGCVHF PHTAPCEVRVLMLLYSSKKKIFMGL IPYDQSGFVNGIRQVITNHKQVQQQ KLEQQQRGMGGQQAPPGLGPILEDQ ARPSQNLLQLRPPQPQPQGTVGASG ATGQPQPQGTAQPPPGAPQGPPGAA SGPPPPGPILRPQNPGANPQLRSLL LNPPPPQTGVPPPQASLHHLQPPGA PALLPPPHQGLGQPQLGPPLLHPPP AQSWPAQLPPRAPLPGQMLLSGGPR GPVPQPGLQPSVMEDDILMDLI
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: MED25 mediator complex subunit 25 [ Homo sapiens ]
• Official Symbol: MED25
• Synonyms: MED25; mediator complex subunit 25; mediator of RNA polymerase II transcription, subunit 25 homolog (S. cerevisiae); mediator of RNA polymerase II transcription subunit 25; ACID1; ARC92; DKFZp434K0512; TCBAP0758; prostate-derived protein 78 kDa; activator-recruited cofactor 92 kDa component; ARC/mediator transcriptional coactivator subunit; activator interaction domain-containing protein 1; mediator of RNA polymerase II transcription, subunit 25 homolog; P78; PTOV2; CMT2B2; MGC70671
• Gene ID: 81857
• mRNA Refseq: NM_030973
• Protein Refseq: NP_112235
• MIM: 610197
• UniProt ID: Q71SY5