Recombinant Full Length Human NCBP1 Protein, C-Flag-tagged

• Cat.No.: NCBP1-1356HFL
• Product Overview: Recombinant Full Length Human NCBP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 91.7 kDa
• AA Sequence: MSRRRHSDENDGGQPHKRRKTSDAN ETEDHLESLICKVGEKSACSLESNL EGLAGVLEADLPNYKSKILR LLCTVARLLPEKLTIYTTLVGLLNA RNYNFGGEFVEAMIRQLKESLKANN YNEAVYLVRFLSDLVNCHVI AAPSMVAMFENFVSVTQEEDVPQVR RDWYVYAFLSSLPWVGKELYEKKDA EMDRIFANTESYLKRRQKTH VPMLQVWTADKPHPQEEYLDCLWAQ IQKLKKDRWQERHILRPYLAFDSIL CEALQHNLPPFTPPPHTEDS VYPMPRVIFRMFDYTDDPEGPVMPG SHSVERFVIEENLHCIIKSHWKERK TCAAQLVSYPGKNKIPLNYH IVEVIFAELFQLPAPPHIDVMYTTL LIELCKLQPGSLPQVLAQATEMLYM RLDTMNTTCVDRFINWFSHH LSNFQFRWSWEDWSDCLSQDPESPK PKFVREVLEKCMRLSYHQRILDIVP PTFSALCPANPTCIYKYGDE SSNSLPGHSVALCLAVAFKSKATND EIFSILKDVPNPNQDDDDDEGFSFN PLKIEVFVQTLLHLAAKSFS HSFSALAKFHEVFKTLAESDEGKLH VLRVMFEVWRNHPQMIAVLVDKMIR TQIVDCAAVANWIFSSELSR DFTRLFVWEILHSTIRKMNKHVLKI QKELEEAKEKLARQHKRRSDDDDRS SDRKDGVLEEQIERLQEKVE SAQSEQKNLFLVIFQRFIMILTEHL VRCETDGTSVLTPWYKNCIERLQQI FLQHHQIIQQYMVTLENLLF
  TAELDPHILAVFQQFCALQATRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Spliceosome

Gene Information

• Gene Name: NCBP1 nuclear cap binding protein subunit 1 [ Homo sapiens (human) ]
• Official Symbol: NCBP1
• Synonyms: NCBP; Sto1; CBP80
• Gene ID: 4686
• mRNA Refseq: NM_002486.5
• Protein Refseq: NP_002477.1
• MIM: 600469
• UniProt ID: Q09161