Recombinant Full Length Human NOS3 Protein, C-Flag-tagged

• Cat.No.: NOS3-157HFL
• Product Overview: Recombinant Full Length Human NOS3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 133.1 kDa
• AA Sequence: MGNLKSVAQEPGPPCGLGLGLGLGL CGKQGPATPAPEPSRAPASLLPPAP EHSPPSSPLTQPPEGPKFP RVKNWEVGSITYDTLSAQAQQDGPC TPRRCLGSLVFPRKLQGRPSPGPPA PEQLLSQARDFINQYYSSI KRSGSQAHEQRLQEVEAEVAATGTY QLRESELVFGAKQAWRNAPRCVGRI QWGKLQVFDARDCRSAQEM FTYICNHIKYATNRGNLRSAITVFP QRCPGRGDFRIWNSQLVRYAGYRQQ DGSVRGDPANVEITELCIQ HGWTPGNGRFDVLPLLLQAPDEPPE LFLLPPELVLEVPLEHPTLEWFAAL GLRWYALPAVSNMLLEIGG LEFPAAPFSGWYMSTEIGTRNLCDP HRYNILEDVAVCMDLDTRTTSSLWK DKAAVEINVAVLHSYQLAK VTIVDHHAATASFMKHLENEQKARG GCPADWAWIVPPISGSLTPVFHQEM VNYFLSPAFRYQPDPWKGS AAKGTGITRKKTFKEVANAVKISAS LMGTVMAKRVKATILYGSETGRAQS YAQQLGRLFRKAFDPRVLC MDEYDVVSLEHETLVLVVTSTFGNG DPPENGESFAAALMEMSGPYNSSPR PEQHKSYKIRFNSISCSDP LVSSWRRKRKESSNTDSAGALGTLR FCVFGLGSRAYPHFCAFARAVDTRL EELGGERLLQLGQGDELCG QEEAFRGWAQAAFQAACETFCVGED AKAAARDIFSPKRSWKRQRYRLSAQ AEGLQLLPGLIHVHRRKMF QATIRSVENLQSSKSTRATILVRLD TGGQEGLQYQPGDHIGVCPPNRPGL VEALLSRVEDPPAPTEPVA VEQLEKGSPGGPPPGWVRDPRLPPC TLRQALTFFLDITSPPSPQLLRLLS TLAEEPREQQELEALSQDP RRYEEWKWFRCPTLLEVLEQFPSVA LPAPLLLTQLPLLQPRYYSVSSAPS THPGEIHLTVAVLAYRTQD GLGPLHYGVCSTWLSQLKPGDPVPC FIRGAPSFRLPPDPSLPCILVGPGT GIAPFRGFWQERLHDIESK GLQPTPMTLVFGCRCSQLDHLYRDE VQNAQQRGVFGRVLTAFSREPDNPK TYVQDILRTELAAEVHRVL CLERGHMFVCGDVTMATNVLQTVQR ILATEGDMELDEAGDVIGVLRDQQR YHEDIFGLTLRTQEVTSRI
  RTQSFSLQERQLRGAVPWAFDPPGS DTNSPTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway

Gene Information

• Gene Name: NOS3 nitric oxide synthase 3 [ Homo sapiens (human) ]
• Official Symbol: NOS3
• Synonyms: eNOS; ECNOS
• Gene ID: 4846
• mRNA Refseq: NM_000603.5
• Protein Refseq: NP_000594.2
• MIM: 163729
• UniProt ID: P29474