Recombinant Full Length Human NRF1 Protein, C-Flag-tagged

• Cat.No.: NRF1-564HFL
• Product Overview: Recombinant Full Length Human NRF1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 53.4 kDa
• AA Sequence: MEEHGVTQTEHMATIEAHAVAQQVQ QVHVATYTEHSMLSADEDSPSSPED TSYDDSDILNSTAADEVTAH LAAAGPVGMAAAAAVATGKKRKRPH VFESNPSIRKRQQTRLLRKLRATLD EYTTRVGQQAIVLCISPSKP NPVFKVFGAAPLENVVRKYKSMILE DLESALAEHAPAPQEVNSELPPLTI DGIPVSVDKMTQAQLRAFIP EMLKYSTGRGKPGWGKESCKPIWWP EDIPWANVRSDVRTEEQKQRVSWTQ ALRTIVKNCYKQHGREDLLY AFEDQQTQTQATATHSIAHLVPSQT VVQTFSNPDGTVSLIQVGTGATVAT LADASELPTTVTVAQVNYSA VADGEVEQNWATLQGGEMTIQTTQA SEATQAVASLAEAAVAASQEMQQGA TVTMALNSEAAAHAVATLAE ATLQGGGQIVLSGETAAAVGALTGV QDANGLVQIPVSMYQTVVTSLAQGN GPVQVAMAPVTTRISDSAVT
  MDGQAVEVVTLEQTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease

Gene Information

• Gene Name: NRF1 nuclear respiratory factor 1 [ Homo sapiens (human) ]
• Official Symbol: NRF1
• Synonyms: ALPHA-PAL
• Gene ID: 4899
• mRNA Refseq: NM_001040110.2
• Protein Refseq: NP_001035199.1
• MIM: 600879
• UniProt ID: Q16656